Anti cd45 ko

Manufactured by Beckman Coulter

Anti-CD45-KO is a laboratory reagent that can be used to detect and identify cells expressing the CD45 antigen. It is a monoclonal antibody conjugated with a fluorescent dye, allowing for flow cytometric analysis of CD45-positive cells. The core function of this product is to provide a tool for researchers to investigate cell surface expression of the CD45 marker.

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Lab products found in correlation

Gallios cytometer, by Beckman Coulter (1 mentions) Anti cd3 ecd, by Beckman Coulter (1 mentions) Anti nkg2c pe, by R&D Systems (1 mentions) Anti cd16 pc7, by Beckman Coulter (1 mentions) Anti cd14 fitc, by Beckman Coulter (1 mentions)

2 protocols using anti cd45 ko

Multiparametric Flow Cytometry of PBMCs

Cited 1 time

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PBMC were stained using the appropriate cocktail of antibodies, as described [14 (link)]: anti-CD45-KO (J33), anti-CD3-ECD (UCHT1), anti-CD56-PC7 (N901), anti-NKG2A-APC (Z199), anti-CD57-PB (NC1), anti-NKp44-PE (Z231), anti-CD16-PB (3G8), anti-ILT-2-PE (HP-F1), from Beckman coulter; anti-KIR2DL2/3-FITC (CH-L), anti-CD69-APC-Cy7 (FN50), anti-CD161-FITC (DX12), anti-HLA-DR-Alexa Fluor 700 (G46-6) from Becton Dickinson; anti-NKp30-APC (AF29-4D12) from Miltenyi Biotec; anti-NKG2C-PE (134591) from R&D Systems. Isotype-matched immunoglobulins served as negative controls. Cells were gated on the CD45⁺ lymphocytes gate. At least 20,000 CD45⁺ cells were analyzed on a Gallios cytometer (Beckman coulter). Flow cytometry data was analyzed using FlowJo software version 9.

Petitdemange C., Wauquier N., Devilliers H., Yssel H., Mombo I., Caron M., Nkoghé D., Debré P., Leroy E, & Vieillard V. (2016). Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients. PLoS Neglected Tropical Diseases, 10(3), e0004499.

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Comprehensive Immune Cell Profiling

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Isolated PBMCs from three different donors were characterized using a CytoDiff flow cytometric system (Navios). Brifely, PBMCs were kept in PBS with 0.1% HSA and differential count of leukocytes was performed after staining with the following antibodies (all from BeckmanCoulter): anti-CD2-APC-AF750 (#B01681), anti-CD14-FITC (#B36297), anti-CD16-PC7 (#6,607,118), anti-CD19-PC5.5 (#B49211), anti-CD34-ECD (#B49202), anti-CD45-KO (#B36294), anti-CD6-PC7 (#A21692), and anti-CD294-PE (#A07413).

Selvin T., Berglund M., Lenhammar L., Jarvius M., Nygren P., Fryknäs M., Larsson R, & Andersson C.R. (2023). Phenotypic screening platform identifies statins as enhancers of immune cell-induced cancer cell death. BMC Cancer, 23, 164.

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