TNM-FH insect medium and Fetal Bovine Serum (FBS) were purchased through Gemini Bio-products (Sacramento, CA). Hi Grace’s Insect Medium (Hi-GI) was purchased through Hi-Media (Mumbai, India). Citric acid was purchased through Fisher Scientific (Pittsburgh, PA), L-arginine hydrochloride through Calbiochem (San Diego, CA), L-lysine monohydrochloride through Acros-organics (Geel, Belgium), and methionine sulfoxide through Alfa Aesar (Haverhill, MA). All other reagents were obtained through Sigma-Aldrich (St. Louis, MO), unless specified. BM-7 and M15 media were prepared as described previously [8 (link), 10 (link)]. For M15 preparation, most components were dissolved with gentle stirring, except for L-tyrosine and three non-water soluble vitamins—biotin, folic acid and riboflavin. L-tyrosine was dissolved in 15 ml of 2 mM HCl prior to addition to M15. Biotin was dissolved in dimethyl sulfoxide, folic acid in 0.1 M NaOH, and riboflavin in 0.01 M NaOH. DL-serine of M15 was replaced by L-serine. Hi-Gi medium was prepared according to the manufacturer instructions. Briefly, 45.9 g of Hi-GI were dissolved in 950 mL of sterile, deionized water, slowly adjusting the pH to 5.92 by titration with 5 M KOH. After completing to 1 L, Hi-GI was sterilized through a 0.22 μm sterile filter. L. crescens strain BT-1 was used in all experiments.
L lysine monohydrochloride
Manufactured by Thermo Fisher Scientific
L-lysine monohydrochloride is a pure chemical compound used as a raw material in various applications. It serves as a building block for the synthesis of other chemicals and pharmaceuticals. The product is offered in a solid, crystalline form.
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Lab products found in correlation
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Citric acid, by Thermo Fisher Scientific (1 mentions)
Tnm fh insect medium, by Gemini Bio (1 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
D glucose, by Thermo Fisher Scientific (1 mentions)
Tween 80, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Honeywell (1 mentions)
Linalool, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Sodium acetate trihydrate, by Merck Group (1 mentions)
Linalool, by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid solution, by Merck Group (1 mentions)
Dipotassium hydrogen phosphate, by Merck Group (1 mentions)
Anise oil, by Merck Group (1 mentions)
Thymol, by AppliChem (1 mentions)
Phthaldialdehyde, by Merck Group (1 mentions)
Tween 80, by Serva Electrophoresis (1 mentions)
Boric acid, by Honeywell (1 mentions)
2 protocols using l lysine monohydrochloride
1
Insect Cell Culture Protocol
2
Aniseed Oil and Thymol Solubilization in Phosphate Buffer
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D-Glucose, L-Lysine monohydrochloride and linalool were purchased from Acros Organics, thymol from Applichem and anise oil from Sigma Aldrich (CAS: 8007-70-3, nature identical, synonym: aniseed oil). All solutions (glucose, lysine and the test compounds) were prepared in phosphate buffer 0.1 M, pH 7.2 ± 0.1 (PBS, Bectron, Dickinson and Company, Bergen County, NJ, USA) and Tween 80 (Serva Electrophoresis GmbH, Heidelberg, Germany) which helps to incorporate aniseed oil and thymol in phosphate buffer. As linalool possesses a very good aqueous solubility at pH 7.0, the use of Tween 80 was not necessary [26] (link).
2-Mercaptoethanol (MCE ≥ 99.0%), phthaldialdehyde (OPA ≥ 99.0%) and hydrochloric acid solution (0.1 N) were purchased from Sigma Aldrich (Steinheim, Germany). Sodium hydroxide (≥98%) and boric acid (≥99.5%) were purchased from Honeywell International Inc (Muskegon, MI, USA). Di-potassium hydrogen phosphate, potassium dihydrogen phosphate and sodium acetate trihydrate were purchased from MERCK (Darmstadt, Germany). Water, methanol and acetonitrile (HPLC grade) were purchased from Fisher Scientific (Loughborough, UK).
2-Mercaptoethanol (MCE ≥ 99.0%), phthaldialdehyde (OPA ≥ 99.0%) and hydrochloric acid solution (0.1 N) were purchased from Sigma Aldrich (Steinheim, Germany). Sodium hydroxide (≥98%) and boric acid (≥99.5%) were purchased from Honeywell International Inc (Muskegon, MI, USA). Di-potassium hydrogen phosphate, potassium dihydrogen phosphate and sodium acetate trihydrate were purchased from MERCK (Darmstadt, Germany). Water, methanol and acetonitrile (HPLC grade) were purchased from Fisher Scientific (Loughborough, UK).
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