Pro il 1β

Manufactured by Thermo Fisher Scientific

Sourced in United States

Pro-IL-1β is a recombinant human protein that represents the precursor form of interleukin-1 beta (IL-1β). It is a member of the interleukin-1 cytokine family and plays a key role in the regulation of immune and inflammatory responses.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Rnaimax, by Thermo Fisher Scientific (1 mentions) Nigericin, by Merck Group (1 mentions) Caspase 1 p10 sc514, by Santa Cruz Biotechnology (1 mentions) Ultrapure lps, by InvivoGen (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) β actin c4, by Santa Cruz Biotechnology (1 mentions) Mouse il 1β, by R&D Systems (1 mentions) Leu leu ome hcl, by Chem-Impex (1 mentions) Ca 074me, by Enzo Life Sciences (1 mentions) Z vad fmk, by Enzo Life Sciences (1 mentions) Endo porter, by Gene Tools (1 mentions) Dy401, by R&D Systems (1 mentions)

Spelling variants of this product

IL-1β (1245 citations) Anti-IL-1β pro-form (2 citations) PE anti-pro-IL-1β (NJTEN3 clone, (2 citations) Anti-mouse Pro-IL-1β APC (2 citations)

2 protocols using pro il 1β

Western Blot Antibodies and ELISA Kits for Studying Inflammation

Cited 1 time

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Antibodies for Western Blots were against mouse IL-1β (R&D Systems), caspase-1 p10 (sc-514; Santa Cruz Biotechnology), NLRP3 (Cryo2; Enzo Life Sciences), β-actin (C4; Santa Cruz Biotechnology) and GAPDH (6C5; EMD Millopore). ELISA kits were purchased for mouse IL-1β (BD Biosciences), pro-IL-1β and TNF-α (eBioscience). Ultrapure LPS was from Salmonella minnesota (Invivogen). Poly(deoxyadenylic-deoxythymidylic) acid and nigericin were purchased from Sigma-Aldrich (St. Louis, MO). Silica crystals (MIN- U-SIL 15) were obtained from U.S. Silica (Frederick, MD). Cholesterol crystals were synthesized by acetone supersaturation and cooling (6 (link)), Alum (Imject alum adjuvant; a mixture of aluminum hydroxide and magnesium hydroxide) was from Pierce Biotechnology, and Leu-Leu-OMe-HCl was from Chem-Impex International. ZVAD-FMK and Ca-074-Me were from Enzo Life Sciences and K777 was initially gifted to us by Stephanie A. Robertson and James H. McKerrow at UCSF, and further stocks obtained through services from the NHLBI’s SMARTT Program. Lipofectamine 2000, RNAiMax and all siRNA smart pools were from Life Technologies and Endoporter was from Gene Tools.

Orlowski G.M., Colbert J.D., Sharma S., Bogyo M., Robertson S.A, & Rock K.L. (2015). Multiple Cathepsins Promote Pro-IL-1β Synthesis and NLRP3-Mediated IL-1β Activation. Journal of immunology (Baltimore, Md. : 1950), 195(4), 1685-1697.

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Measuring Inflammatory Cytokines: ELISA Protocols

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ELISA kits were used to measure IL-1β, IL-1α, IL-33 (DY401, R&D Systems, Wiesbaden-Nordenstadt, Germany), HMGB1 (Cedarlane Laboratories USA Inc., Burlington, USA) and pro-IL-1β (e-Biosciences). Assays were run according to the manufacturer's protocols with a detection limit of 5 pg/ml for IL-1β, IL-1α, IL-33, 0,2 ng/ml for HMGB1 and 25 pg/ml for pro-IL-1β

Rabolli V., Badissi A.A., Devosse R., Uwambayinema F., Yakoub Y., Palmai-Pallag M., Lebrun A., De Gussem V., Couillin I., Ryffel B., Marbaix E., Lison D, & Huaux F. (2014). The alarmin IL-1α is a master cytokine in acute lung inflammation induced by silica micro- and nanoparticles. Particle and Fibre Toxicology, 11, 69.

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