The UV-vis spectrum of nanorod aqueous solution (Figure 1 ) was acquired with an Agilent/HP 8453 UV-vis spectrophotometer (200 nm < λ < 1100 nm, Dublin, Ireland). Extinction spectrum of gold nanorods deposited on glass coverslips (Figure 1 ) was acquired with an inverted IX-71 Olympus microscope under halogen lamp (100 W) illumination. The spectrum was acquired by directing the light collected by the objective into the entrance of slit of a monochromator (SP-300i, Acton Research, UK) equipped with a thermoelectrically cooled, back illuminated CCD (Spec10:100B, Princeton Instruments, UK).
Spec 10 100b
Manufactured by Teledyne
Sourced in United States
The Spec-10: 100B is a back-illuminated, high-performance CCD (Charge-Coupled Device) camera system. It features a 1340 x 100 pixel sensor with a pixel size of 20 x 20 microns. The camera is designed for spectroscopy and low-light imaging applications.
Automatically generated - may contain errors
5 protocols using spec 10 100b
1
Optical Characterization of Gold Nanorods
2
Raman Spectroscopy of Supernatants
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Spontaneous
Raman spectroscopy was performed as described elsewhere.39 (link),40 (link) Briefly, a continuous-wave laser at 532 nm (Verdi V5, Coherent)
was the excitation light source. Backscattered Raman light from the
supernatants in a quartz cuvette (10 mm × 10 mm) was collected,
dispersed, and recorded by a high-resolution monochromator (Triple-Pro,
Acton Research) coupled with a liquid-nitrogen-cooled CCD camera (Spec-10:100B,
Princeton Instruments). The acquisition time for each measurement
was 1 min, and the average of 10 acquisitions was used to achieve
better signal-to-noise ratios in Raman spectra. Additionally, the
Raman spectra of water were recorded in identical conditions. After
subtracting the background spectra of the solvent, the revised Raman
spectra were reported and analyzed, providing sufficiently reliable
data for kinetic analyses. The resolution of the present Raman spectra
was ∼1 cm–1 in the frequency range of 200–3000
cm–1, and the wavelength was calibrated using standard
spectral lines from a mercury lamp.
Raman spectroscopy was performed as described elsewhere.39 (link),40 (link) Briefly, a continuous-wave laser at 532 nm (Verdi V5, Coherent)
was the excitation light source. Backscattered Raman light from the
supernatants in a quartz cuvette (10 mm × 10 mm) was collected,
dispersed, and recorded by a high-resolution monochromator (Triple-Pro,
Acton Research) coupled with a liquid-nitrogen-cooled CCD camera (Spec-10:100B,
Princeton Instruments). The acquisition time for each measurement
was 1 min, and the average of 10 acquisitions was used to achieve
better signal-to-noise ratios in Raman spectra. Additionally, the
Raman spectra of water were recorded in identical conditions. After
subtracting the background spectra of the solvent, the revised Raman
spectra were reported and analyzed, providing sufficiently reliable
data for kinetic analyses. The resolution of the present Raman spectra
was ∼1 cm–1 in the frequency range of 200–3000
cm–1, and the wavelength was calibrated using standard
spectral lines from a mercury lamp.
3
Micro-Raman and XPS Analysis of Implant
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Micro-Raman spectra were obtained by using an NRS-2000C (Jasco International Co. Ltd., Tokyo, Japan) instrument with a microscope of 100× original magnification. All the spectra were recorded in back-scattering conditions with 5 cm−1 spectral resolution by using the 532 nm green diode-pumped solid-state laser driver (RgBLase LLC, Fremont, CA, USA). A 160 K cooled digital charge coupled device (Spec-10: 100B, Roper Scientific Inc., Sarasota, FL, USA) was used as a detector. Laser power on the sample was about 10 mW for the implant before immersion and about 20 mW for the HBSS-aged sample. To obtain a good representation of the analyzed implants, 8–10 micro-Raman spectra were collected in different points of each sample.
XPS was performed on the implant before and after immersion for 28 days in HBSS to investigate the surface modification and apatite nucleation ability. A hemispherical energy analyzer (9 channeltron Phoibos HSA3500 150, SPECS GmbH, Berlin, Germany) was used. X-ray source was MgKα emission line (1253.6 eV) with an incidence of 45°. Samples were analyzed without any treatment at 7 × 10−10 mbar pressure. Data were acquired with LabSpecs and analyzed with Igor Pro 6.37 software.
XPS was performed on the implant before and after immersion for 28 days in HBSS to investigate the surface modification and apatite nucleation ability. A hemispherical energy analyzer (9 channeltron Phoibos HSA3500 150, SPECS GmbH, Berlin, Germany) was used. X-ray source was MgKα emission line (1253.6 eV) with an incidence of 45°. Samples were analyzed without any treatment at 7 × 10−10 mbar pressure. Data were acquired with LabSpecs and analyzed with Igor Pro 6.37 software.
4
Characterization of Functionalized MnGC
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Raman spectra were registered on a Jasco NRS-2000C spectrometer equipped with a microscope with 100× magnification. The spectra were recorded under backscattering conditions with 4 cm−1 spectral resolution using the 532 nm line (DPSS laser driver, LGBlase LLC, Fremont, CA, USA) with a power of about 20 mW. The detector was a liquid nitrogen cooled CCD (Spec-10: 100B, Roper Scientific, Inc., Tucson, AZ, USA). Each spectrum was the average of 16 measurements.
IR spectra were recorded with a Bruker ALPHA series FT-IR spectrometer (Bruker, Ettlingen, Germany) equipped with an attenuated total reflectance (diamond crystal) apparatus and a Deuterated Lanthanum α-Alanine doped TriGlycine Sulfate (DLaTGS) detector. The spectra were averaged over 100 scans at a resolution of 4 cm−1.
The morphology of both MnGC and BMP-MnGC has been investigated by a Scanning Electron Microscopy (SEM). The machine employed is a COXEM EM 30AX plus equipped with a Tungsten Filament (W), a SE Detector, and BSE Detector (Solid type 4 Channel).
The success of the functionalization process has been tested through an elemental analysis with SEM-EDX, i.e., the aforementioned SEM equipped with an energy dispersive X-ray detector (EDX, model EDAX Element-C2B).
IR spectra were recorded with a Bruker ALPHA series FT-IR spectrometer (Bruker, Ettlingen, Germany) equipped with an attenuated total reflectance (diamond crystal) apparatus and a Deuterated Lanthanum α-Alanine doped TriGlycine Sulfate (DLaTGS) detector. The spectra were averaged over 100 scans at a resolution of 4 cm−1.
The morphology of both MnGC and BMP-MnGC has been investigated by a Scanning Electron Microscopy (SEM). The machine employed is a COXEM EM 30AX plus equipped with a Tungsten Filament (W), a SE Detector, and BSE Detector (Solid type 4 Channel).
The success of the functionalization process has been tested through an elemental analysis with SEM-EDX, i.e., the aforementioned SEM equipped with an energy dispersive X-ray detector (EDX, model EDAX Element-C2B).
5
Evaluating Aldehyde Peptide Anchoring by Raman and IR
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Vibrational Raman and IR spectroscopies was used to assess the efficacy of the novel anchoring strategy via aldehyde peptide. Raman spectra were registered on a Jasco NRS-2000C spectrometer equipped with a microscope with 100× magnification. The spectra were recorded under backscattering conditions with 4 cm−1 spectral resolution using the 532 nm line (DPSS laser driver, LGBlase LLC, Fremont, CA, USA) with a power of about 2 mW. The detector was a liquid nitrogen-cooled CCD (Spec-10:100B, Roper Scientific, Inc., Tucson, AZ, USA). Each spectrum was the average of three measurements recorded at three different points of each sample.
IR spectra were recorded in triplicate on a Shimadzu IRTracer-100 Fourier Transform FTIR spectrometer equipped with a QATR-10 single crystal diamond Attenuated Total Reflectance (ATR) accessory and a Deuterated Lanthanum α-Alanine doped TriGlycine Sulphate (DLaTGS) detector; the spectral resolution was 4 cm−1 with 64 scans for each spectrum.
IR spectra were recorded in triplicate on a Shimadzu IRTracer-100 Fourier Transform FTIR spectrometer equipped with a QATR-10 single crystal diamond Attenuated Total Reflectance (ATR) accessory and a Deuterated Lanthanum α-Alanine doped TriGlycine Sulphate (DLaTGS) detector; the spectral resolution was 4 cm−1 with 64 scans for each spectrum.
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