Timed-pregnant C57BL/6 mice were purchased from Charles River Laboratories. Biochemicals included MK-801, D-serine, glycine and lithium (Sigma), 7-chlorokynurenic acid (7-CK) (Tocris Bioscience). Antibodies included α-PSD-95 (BD Transduction Laboratories, 1:1000 for WB; NeuroMab, 75–028, 1:150 for ICC)[20 (link), 29 (link)], α-DISC1 (Millipore, ABN425, 1:250 for ICC; 1:1000 for WB), α-serine racemase (Abcam, ab45434, 1:250 for ICC, 1:1000 for WB, 2.5μg for IP) [20 (link)], α-actin (Abcam, ab3280, 1:5000 for WB)[20 (link), 28 (link), 29 (link), 50 (link)]
7 chlorokynurenic acid 7 ck
Manufactured by Bio-Techne
7-chlorokynurenic acid (7-CK) is a chemical compound that functions as a selective antagonist of the glycine binding site of the N-methyl-D-aspartate (NMDA) receptor. It is used as a research tool in the study of NMDA receptor function and neurological processes.
Automatically generated - may contain errors
Lab products found in correlation
D serine, by Merck Group (2 mentions)
α psd 95, by BD (2 mentions)
Glycine, by Merck Group (2 mentions)
C57bl 6 mice, by Charles River Laboratories (2 mentions)
Ab45434, by Abcam (1 mentions)
Ab3280, by Abcam (1 mentions)
Mk 801, by Merck Group (1 mentions)
2 amino 5 phosphonopentanoic acid ap5, by Merck Group (1 mentions)
α vglut1, by Synaptic Systems (1 mentions)
α gfap, by Merck Group (1 mentions)
α map2, by Abcam (1 mentions)
α gaba, by Merck Group (1 mentions)
L serine, by Merck Group (1 mentions)
2 protocols using 7 chlorokynurenic acid 7 ck
1
Timed-Pregnant Mouse Model Biochemicals
2
Immunostaining of Synaptic Proteins
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Timed-pregnant C57BL/6 mice were purchased from Charles River Laboratories. Biochemicals included 2-amino-5-phosphonopentanoic acid (AP5), D-serine, L-serine, glycine and lithium (Sigma), 7-chlorokynurenic acid (7-CK) (Tocris Bioscience). Antibodies included α-NR1 (BD Pharmagen, mouse monoclonal, Millipore, mouse monoclonal), α-NR2A, α-NR2B (Alomone Labs, rabbit polyclonal), α-PSD-95 (BD Transduction Laboratories, mouse monoclonal; NeuroMab, mouse monoclonal), α-VGLUT1 (Synaptic Systems, polyclonal; mouse monoclonal), α-GAD65 (DSHB, mouse monoclonal), α-gephyrin (Synaptic systems, mouse monoclonal), α-D-serine, α-L-serine, α-Serine Racemase (Abcam, rabbit polyclonal), α-GFAP (Sigma, rabbit polyclonal; NeuroMab, mouse monoclonal), α-GLT1 (obtained from Dr. Michael B. Robinson, a courtesy of Dr. Jeffrey Rothstein, mouse monoclonal; NeuroMab, rabbit polyclonal), α-MAP2 (Abcam, chicken polyclonal), α-GABA (Sigma, rabbit polyclonal), α-VAMP2 (Synaptic systems, mouse monoclonal).
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