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2 protocols using agi 1067

1

Targeting cellular pathways with inhibitors

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XL388 was provided by Dr. Zhang [24 (link)] at Southeast University of China. N-acetylcysteine (NAC), pyrrolidine dithiocarbamate (PDTC), AGI-1067, puromycin, rapamycin, perifosine and LY294002 were obtained from Sigma-Aldrich (St. Louis, MO). Cell culture reagents were purchased from Gibco-BRL Co. (Grand Island, NY). Antibodies utilized in this study were provided by Cell Signaling Tech (Shanghai, China) and Abcam Co. (Beijing, China). TRIzol and other reagents for RNA assays as well as Lipofectamine 2000 and other transfection reagents were provided by Thermo-Fisher Invitrogen Co. (Shanghai, China).
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2

Antioxidant Effects on Cell Viability

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CCK-8 was obtained from Dojindo (Kumamoto, Japan). Antioxidants including NAC, PDTC, and AGI-1067, as well as puromycin, neomycin, polybrene, and Matrigel were provided by Sigma-Aldrich Chemicals (St. Louis, MO, USA). Cell culture reagents, including fetal bovine serum (FBS), DMEM, and antibiotics, were obtained from Hyclone (Logan, UT, USA). Antibodies of MAFG, HMOX1, and tubulin were provided by Cell Signaling Technology (Beverly, MA, USA).
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