CUR was purchased from Shanghai D&B biological science and technology Co., Ltd. (Shanghai, China). PEG5k-b-PPLA5k was purchased from Jinan Daigang Biomaterial Co., Ltd. (Jinan, China). MDA-MB-231 cells were obtained from the ATCC (Manassas, VA, USA). MTT was supplied by Sigma (St. Louis, MO, USA). Rabbit anti-human anti-STAT3 (BM4052), anti-phospho-STAT3 (Tyr705) (BM4835), anti-JAK2 (BM4165), and anti-phospho-JAK2 (Tyr1007/Tyr1008) (BM4839) antibody products were obtained from Boster Biological Technology Co., Ltd. (Wuhan, China). HRP-labeled Goat Anti-Rabbit IgG (A0208) and the Annexin V-FITC apoptosis detection kit (C1062M) were purchased from Beyotime Biotechnology (Shanghai, China). 24-well transwell chambers (Cat. No. 3422) and polymerized Matrigel (Cat. No. 356234) were bought from Corning (Corning, NY, USA).
Annexin 5 fitc apoptosis detection kit c1062m
Manufactured by Beyotime
Sourced in China
The Annexin V-FITC Apoptosis Detection Kit (C1062M) is a laboratory reagent designed to detect and quantify apoptosis, a programmed cell death process. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein, conjugated to the fluorescent dye FITC, to identify cells undergoing apoptosis. This system provides a straightforward method for measuring apoptosis in cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 231 cell, by American Type Culture Collection (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Hrp labeled goat anti rabbit igg a0208, by Beyotime (1 mentions)
Cell cycle and apoptosis analysis kit, by Beyotime (1 mentions)
Polyvinylidene fluoride (pvdf), by Merck Group (1 mentions)
Mek162, by CSNpharm (1 mentions)
Ham s f12k kaighn s medium f12k, by Thermo Fisher Scientific (1 mentions)
Fast silver stain kit, by Beyotime (1 mentions)
Cell counting kit 8 cck 8, by Targetmol (1 mentions)
Super ecl detection reagent, by Yeasen (1 mentions)
Venetoclax abt 199, by Selleck Chemicals (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Mcl 1, by Affinity Biosciences (1 mentions)
Mg132, by Selleck Chemicals (1 mentions)
3 protocols using annexin 5 fitc apoptosis detection kit c1062m
1
Cell Migration and Apoptosis Assay Protocol
2
Cucurbitacin B and MEK162 Protocol
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Cucurbitacin B (98%) was purchased from the Nanjing SenBeiJia Biological Technology Co., Ltd. (SBJ-I0498, Jiangsu, China). MEK162 was purchased from the CSNpharm (CSN16001, Chicago, USA). Fetal bovine serum (FBS, 42F6590K), RPMI-1640 medium HEPES (1640H, 22400089) and Ham's F12K (Kaighn's) medium (F12K, 21127022) were from Gibco (NY, USA). Phosphate buffer solution (PBS), phenylmethanesulfonyl fluoride (PMSF, 20104ES03), RIPA lysis buffer (20114ES60), penicillin–streptomycin (60162ES76), Super ECL Detection Reagent (36208ES60) were from Yeasen (Shanghai, China). Serum-free cell freezing medium was from NCM Biotech Co., Ltd. (C40050, Suzhou, China). Cell counting kit-8 (CCK-8) was procured from TargetMol (C0005, Boston, USA). Cell Cycle and Apoptosis Analysis Kit (C1052), Annexin V-FITC Apoptosis Detection Kit (C1062M) and Fast Silver Stain Kit (P0017S) were procured from Beyotime Biotechnology (Jiangsu, China). Multicolor protein markers were from Biorad (161-0394, CA, USA) and Yeasen (20352ES76, Shanghai, China). Polyvinylidene fluoride (PVDF) was from Merck Millipore (IPVH00010, Darmstadt, Germany).
3
Venetoclax and Metformin Cytotoxicity Study
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Venetoclax (ABT-199) (1257044-40-8) was purchased from Selleck and Metformin hydrochloride (MB1927-S) was purchased from the Dalian Meilun Biotechnology Co. Cycloheximide was purchased from MCE and MG-132 was obtained from Selleck. Cell Counting Kit (CCK8, B34304) was purchased from Bimake (USA). Annexin V-FITC Apoptosis Detection Kit (C1062M) was purchased from Beyotime (Shang Hai, China). The antibodies β-Actin, BCL-2 and PARP were purchased from Cell Signaling Technology, while BAX and BCL-xl were from ABcone, MCL-1 was purchased from Affinity.
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