Male Long-Evans rats (250 – 300 g, 8 –10 weeks of age; Harlan Laboratories, Indianapolis, IN) were single housed at the Metabolic Diseases Institute of the University of Cincinnati under standard controlled conditions (12:12-h light-dark cycle, 50–60% humidity, 25°C). Animals had free access to water and a high-fat diet (HFD) (40% fat; 4.54 kcal/g, D03082706; Research Diets, New Brunswick, New Jersey) for eight weeks. Three days pre-operatively, rats were matched for body weight and fat mass and randomized to RYGB, VSG, RYGB-sham, and VSG-sham surgical groups (n=6) and the high-fat diet was replaced with Osmolite OneCal liquid diet (Abbott, Columbus, OH). VSG and RYGB surgery were performed in anesthetized rats as described previously (Chambers et al., 2011 (link)). All surgical groups received postoperative care consisting of subcutaneous injections of Metacam (0.25 mg/100 g body weight once daily for 4 days), Buprenex (0.3 mL twice a day for 5 days), and warm saline (10 mL and 5 mL twice daily for days 0–3 and 4–5, respectively). During the 5 days of postoperative care, rats had free access to Osmolite OneCal liquid diet (Abbott, Columbus, OH) until they were switched back to solid HFD diet. All rats were sacrificed 30 days after surgery.
Osmolite onecal liquid diet
Manufactured by Abbott
Sourced in United States
Osmolite OneCal is a liquid diet formula produced by Abbott. It is a nutritionally complete, high-calorie medical nutrition product designed to meet the dietary needs of individuals who require supplemental or sole-source nutrition.
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Lab products found in correlation
4 protocols using osmolite onecal liquid diet
1
Metabolic Effects of Bariatric Surgeries in Rats
2
Metabolic Effects of Bariatric Surgeries in Rats
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Male Long-Evans rats (250 – 300 g, 8 –10 weeks of age; Harlan Laboratories, Indianapolis, IN) were single housed at the Metabolic Diseases Institute of the University of Cincinnati under standard controlled conditions (12:12-h light-dark cycle, 50–60% humidity, 25°C). Animals had free access to water and a high-fat diet (HFD) (40% fat; 4.54 kcal/g, D03082706; Research Diets, New Brunswick, New Jersey) for eight weeks. Three days pre-operatively, rats were matched for body weight and fat mass and randomized to RYGB, VSG, RYGB-sham, and VSG-sham surgical groups (n=6) and the high-fat diet was replaced with Osmolite OneCal liquid diet (Abbott, Columbus, OH). VSG and RYGB surgery were performed in anesthetized rats as described previously (Chambers et al., 2011 (link)). All surgical groups received postoperative care consisting of subcutaneous injections of Metacam (0.25 mg/100 g body weight once daily for 4 days), Buprenex (0.3 mL twice a day for 5 days), and warm saline (10 mL and 5 mL twice daily for days 0–3 and 4–5, respectively). During the 5 days of postoperative care, rats had free access to Osmolite OneCal liquid diet (Abbott, Columbus, OH) until they were switched back to solid HFD diet. All rats were sacrificed 30 days after surgery.
3
Vertical Sleeve Gastrectomy in Rodents
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Four days prior to surgery, body composition was assessed using an EchoMRI analyzer (Houston, TX). Animals were fed Osmolite OneCal liquid diet (Abbott Laboratories, IL) but no solid-food for 24 h prior to surgery.
VSG/Sham-VSG: We followed procedures as previously described [31 (link)]. VSG consisted of a midline abdominal laparotomy with exteriorization of the stomach. Ligaments and connective tissue were removed leaving an easily-articulated stomach. The lateral 80% of the stomach was excised using an ENDO GIA Ultra Universal stapler (#EGIAUSHORT, Covidien, MA) coupled with an ENDO GIA Auto Suture Universal Articulating Loading Unit, 45 mm–2.5 mm (#030454, Covidien, MA). A gastric tube in continuity with the esophagus and duodenum thus remained. This gastric sleeve was then reintegrated into the abdominal cavity and the abdominal wall was closed in layers using 4–0 coated vicryl suture (VE494, Ethicon, OH). The Sham-VSG mimics the VSG surgery including externalization of the stomach. However, in the case of the Sham, the stomach is gently pressed with forceps in the approximate area of the surgical resection and then immediately reintegrated into the abdomen which is then sutured and stapled.
VSG/Sham-VSG: We followed procedures as previously described [31 (link)]. VSG consisted of a midline abdominal laparotomy with exteriorization of the stomach. Ligaments and connective tissue were removed leaving an easily-articulated stomach. The lateral 80% of the stomach was excised using an ENDO GIA Ultra Universal stapler (#EGIAUSHORT, Covidien, MA) coupled with an ENDO GIA Auto Suture Universal Articulating Loading Unit, 45 mm–2.5 mm (#030454, Covidien, MA). A gastric tube in continuity with the esophagus and duodenum thus remained. This gastric sleeve was then reintegrated into the abdominal cavity and the abdominal wall was closed in layers using 4–0 coated vicryl suture (VE494, Ethicon, OH). The Sham-VSG mimics the VSG surgery including externalization of the stomach. However, in the case of the Sham, the stomach is gently pressed with forceps in the approximate area of the surgical resection and then immediately reintegrated into the abdomen which is then sutured and stapled.
4
Vertical Sleeve Gastrectomy in Rats
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Animal care was performed as previously described [22 (link),25 (link),26 (link),27 (link)]. Briefly, animals were maintained on Osmolite OneCal liquid diet (Abbott Laboratories, North Chicago, IL, USA). In order to encourage a clear digestive tract, no-solid diet was provided 24 h before surgery. For VSG surgery, rats were anesthetized with isoflurane (Piramal Enterprises, Ltd., Andhra Pradesh, Telegana, India). Vertical sleeve gastrectomy involved a medial abdominal incision through the skin and muscular layer. The ligaments were cut to produce stomach externalization. To remove the lateral 80% of the stomach, an ENDO GIA Ultra Universal stapler (#EGIAUSHORT, Covidien, Mansfield, MA, USA) with an ENDO GIA Auto Suture Universal Articulating Loading Unit, 45 mm–2.5 mm (#030454, Covidien, Waltham, MA, USA) was used to reconfigure the now tubular stomach. The gastric sleeve was replaced in the abdominal cavity and sutured with 4-0 vicryl suture. For sham VSG, following laparotomy, the stomach was externalized and forcefully pressed between blunt forceps for 15 s, and then reintegrated into the abdominal cavity.
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