Beclin-1 and LC3B rabbit polyclonal antibodies were from Cell Signaling (Danvers, MA). Actin mouse monoclonal antibody was from Chemicon (Billerica, MA). AY9944 (trans-1,4 bis-(2-dichlorobenzyl-aminomethyl)cyclohexane dihydrochloride) (1 μM as working solution), N-acetylcysteine (NAC) and monodansylcadaverine (MDC) were from Sigma (St. Louis, MO). Lipoprotein–deficient serum was from Cocalico Biologicals, Inc. (Reamstown, PA). LC3-GFP was from Addgene and transfast transfection kits were from Promega. Lipofectamine 2000 and the antioxidant cocktail supplement B27 (50 X stock solution) was from Invitrogen (Grand Island, NY. JC-1 Mitochondrial Membrane Potential Assay Kit was from Cayman Chemical Company (Ann Arbor, MI).
Transfast transfection kits
Manufactured by Promega
Transfast transfection kits are laboratory reagents used to introduce genetic material, such as DNA or RNA, into cells. They provide a reliable and efficient method for transfection, which is the process of delivering foreign genetic material into cells. The kits contain a proprietary transfection reagent that facilitates the uptake of the genetic material by the target cells.
Automatically generated - may contain errors
Lab products found in correlation
Monodansylcadaverine mdc, by Merck Group (2 mentions)
N acetylcysteine nac, by Merck Group (2 mentions)
Gfp lc3, by Addgene (2 mentions)
Lc3b rabbit polyclonal antibody, by Cell Signaling Technology (2 mentions)
Antioxidant cocktail supplement b27, by Thermo Fisher Scientific (2 mentions)
Jc 1 mitochondrial membrane potential assay kit, by Cayman Chemical (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
2 protocols using transfast transfection kits
1
Analyzing Autophagy and Mitochondrial Function
2
Autophagy Regulation in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Beclin-1 and LC3B rabbit polyclonal antibodies were from Cell Signaling (Danvers, MA). Actin mouse monoclonal antibody was from Chemicon (Billerica, MA). AY9944 (trans-1,4 bis-(2-dichlorobenzyl-aminomethyl)cyclohexane dihydrochloride) (1 μM as a working solution), N-acetylcysteine (NAC) and monodansylcadaverine (MDC) were from Sigma (St. Louis, MO). Lipoprotein-deficient serum was from Cocalico Biologicals, Inc. (Reamstown, PA). LC3-GFP was from Addgene and transfast transfection kits were from Promega. Lipofectamine 2000 and the antioxidant cocktail supplement B27 (50 × stock solution) were from Invitrogen (Grand Island, NY. JC-1 Mitochondrial Membrane Potential Assay Kit was from Cayman Chemical Company (Ann Arbor, MI).
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