All animal experiments were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals (U.S. Department of Health and Human Services, Public Health Services, National Institute of Health, NIH publication no. 86–23, 1985)34 , and approved by the Ethical Committee for Animal Experiments at the University of Tokyo. EGFP-overexpressing C57BL/6 mice (C57BL/6-Tg (CAG-EGFP)) were purchased from Japan SLC Inc. C3H/HeN and C3H/HeJ mice were purchased from CLEA Japan. All mice were allowed access to feed and water ad libitum.
C3h hen
Manufactured by CLEA Japan
Sourced in Japan
The C3H/HeN is a laboratory equipment product that serves as a mouse strain. It is a genetically-modified mouse model used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using c3h hen
1
Ethical Animal Experiments for EGFP Mice
2
Mice Strains for Immunological Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J, C3H/HeJ and C3H/HeN mice were purchased from Clea Japan (Tokyo, Japan). The genetic background of the genetically engineered mice used was C57BL/6J. Il6−/− (IL-6-KO), Cd19-Cre, Jh−/− (Jh-KO) and C57BL/6-Ly5.1 mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA); mice with homozygous Cd19 deficiency (Cd19cre/cre) were used as Cd19−/− mice. Myd88−/− (Myd88-KO), Ticam−/− (Trif-KO) and Tlr4−/− (TLR4-KO) mice were purchased from Oriental BioService (Kyoto, Japan). Fcα/μR−/− (Fcα/μR-KO) mice were generated in our laboratory, as previously described, and backcrossed onto the C57BL/6 genetic background for 12 generations. Only female mice between the ages of 8 weeks and 12 weeks were used for the experiments. All experiments were performed in accordance with the guidelines of the animal ethics committee of the University of Tsukuba Animal Research Center.
3
Strain-Specific Mouse Acclimatization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Six-week-old female mice from four inbred strains (BALB/c, C57BL/6J, C3H/HeN, DBA/2J) were used (CLEA Japan, Inc., Tokyo, Japan). To eliminate the influence of the rearing environment, mice
were housed in TPX plastic cages (220 × 320 × 135 mm, Natsune Seisakusho, Co., Ltd., Tokyo, Japan) with wire mesh floors for a 2-week acclimatization period. All animals were kept in an
animal room at a constant temperature (23 ± 2°C), humidity (55 ± 10%), and 12 h light/12 h dark cycle (lights on at 07:00 and off at 19:00). The mice were fed a plain commercial diet (NMF,
Oriental Yeast Co., Ltd., Tokyo, Japan) and had tap water ad libitum. All experiments were performed according to the Guide for the Care and Use of Laboratory Animals at
Shimane University and approved by the Institutional Review Board of the University (Approved number: IZ21-127).
were housed in TPX plastic cages (220 × 320 × 135 mm, Natsune Seisakusho, Co., Ltd., Tokyo, Japan) with wire mesh floors for a 2-week acclimatization period. All animals were kept in an
animal room at a constant temperature (23 ± 2°C), humidity (55 ± 10%), and 12 h light/12 h dark cycle (lights on at 07:00 and off at 19:00). The mice were fed a plain commercial diet (NMF,
Oriental Yeast Co., Ltd., Tokyo, Japan) and had tap water ad libitum. All experiments were performed according to the Guide for the Care and Use of Laboratory Animals at
Shimane University and approved by the Institutional Review Board of the University (Approved number: IZ21-127).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!