After antigen repair and blockage of endogenous peroxidases, all sections were incubated with anti-FOXO4 (1 : 50, Abcam) overnight at 4°C and secondary antibody (Maixin, China) was applied at 37°C for 30 min. Finally, slides were evaluated using the brown DAB precipitate (Maixin, China) and stained with hematoxylin. Under the microscope (Olympus BX61, Japan), three regions were randomly selected from all sections for MOD (MOD = integral optical density/measurement area) analysis.
Brown dab precipitate
Manufactured by Maixin Group
Sourced in China
Brown DAB precipitate is a laboratory reagent used to detect the presence of specific proteins or molecules in biological samples. It is a chromogenic substrate that produces a brown-colored precipitate when exposed to the target analyte. The core function of the Brown DAB precipitate is to serve as a visual indicator for the detection and localization of target molecules in various experimental applications, such as immunohistochemistry and Western blotting.
Automatically generated - may contain errors
Lab products found in correlation
Anti foxo4, by Abcam (1 mentions)
Anti cdk2, by Abcam (1 mentions)
Anti cdk4, by Bioss Antibodies (1 mentions)
Anti cdk6, by Bioss Antibodies (1 mentions)
Anti ki67, by Maixin Group (1 mentions)
Anti p21, by Bioss Antibodies (1 mentions)
Anti phb2, by Abcam (1 mentions)
Anti lc3, by Abcam (1 mentions)
3 protocols using brown dab precipitate
1
Immunohistochemical Detection of FOXO4
2
Immunohistochemical Analysis of Signaling Pathways
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immunohistochemistry and histology (IHC) staining was performed to detect the expression of p38MAPK, NFκB, caspase 3, and GFAP. After the antigens were repaired, all sections were incubated with the primary antibody for p38MAPK (1:300, Bioss), NFκB (1:300, Abcam), caspase 3 (1:200, Abcam), and GFAP (1:1000, Proteintech) overnight at 4°C after blockage of endogenous peroxidases. Next, the sections were incubated with the secondary antibody (Maixin, China) at 37°C for 30 min. Finally, slides were evaluated using the brown DAB precipitate (Maixin, China) and were performed with hematoxylin. All sections were observed under the microscope (Olympus BX61, Japan) to randomly select three regions for MOD analysis.
3
Immunohistochemical Analysis of Senescence and Mitophagy
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Immunohistochemistry (IHC) staining was performed to detect the expression of senescence and mitophagy proteins. After antigens repaired, all sections were incubated with the primary antibody overnight at 4°C after blockage of endogenous peroxidases. The primary antibodies were used as follows: anti-Ki67 (Maixin), anti-wtp53 (1:300, Bioss), anti-p21 (1:300, Bioss), anti-CDK2 (1:50, Abcam), anti-CDK4 (1:100, Bioss), anti-CDK6 (1:400, Bioss), anti-PHB2 (1:200, Abcam) and anti-LC3 (1:100, Abcam). Next, the sections were incubated with secondary antibody (Maixin, China) at 37°C for 30 minutes. Finally, slides were evaluated using the brown DAB precipitate (Maixin, China) and were performed with hematoxylin.
All sections were observed under the microscope (Olympus BX61, Japan) to randomly select three regions at 200× magnification. Image-Pro Plus software was used for MOD quantitative analyses (MOD = integral optical density/measurement area).
All sections were observed under the microscope (Olympus BX61, Japan) to randomly select three regions at 200× magnification. Image-Pro Plus software was used for MOD quantitative analyses (MOD = integral optical density/measurement area).
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