FRI was performed using the fDOT prototype using a light-emitting diode (LED) ring at 660 nm (Hamamatsu, Massy, France). Red fluorescence images (exposure time 1 s) were acquired through the RG9 filter with a CCD camera (Hamamatsu, Massy, France). FRI was also performed using a Lumina LT apparatus (Perkin Elmer). Excitation was performed at 675 nm, and fluorescence emission was detected with a 695–770 nm filter. For the in vitro study, U87-iRFP+-Fluc+ cells were plated in 24-well culture plates and scanned using an Odyssey scanner (Li-Core Biosciences, Lincoln, NE, USA) at 700 nm.
Lumina lt apparatus
Manufactured by PerkinElmer
Sourced in United States
The Lumina LT apparatus is a laboratory equipment designed for luminescence-based analysis. It is capable of detecting and measuring light emission from various samples. The core function of the Lumina LT is to provide accurate and reliable luminescence data for research and diagnostic applications.
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Lab products found in correlation
3 protocols using lumina lt apparatus
1
Fluorescence Imaging of Tumor Cells
2
Fluorescence Reflectance Imaging Protocols
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FRI was performed using the Lumina LT apparatus (Perkin Elmer, Boston, MA, USA) with the 745 nm excitation filter and the 810–875 nm emission filter. Fluorescence images (1 s, 4 × 4 binning) and photographs (100 ms) were acquired successively, and analyzed using Living Image software. FRI signal is expressed as photons·s−1·cm−2·sr−1.
FRI was also performed using the per-operatory camera system Fluobeam® (Fluoptics, Grenoble, France) at a spectral window of excitation of 780 nm and with an emission of 820 nm. The image was analyzed using Image J software.
FRI was also performed using the per-operatory camera system Fluobeam® (Fluoptics, Grenoble, France) at a spectral window of excitation of 780 nm and with an emission of 820 nm. The image was analyzed using Image J software.
3
Fluorescence Imaging of Tumor Tissue
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FRI was performed using the Lumina LT apparatus (Perkin Elmer). Excitation was performed at 745 nm and fluorescence emission was detected with the 810-875 nm filter. A region of interest was placed on the tumor to allow for the quantification of fluorescence signal (ph.s -1 ) using Living Image software.
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