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Plasmid midiprep system

Manufactured by Promega
Sourced in Germany

The Plasmid Midiprep System is a laboratory equipment product designed for the purification of plasmid DNA from bacterial cultures. It is a automated system that efficiently extracts and purifies plasmid DNA for various applications in molecular biology and genetic research.

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2 protocols using plasmid midiprep system

1

Plasmid Transfection and Cell Assays

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Cell lines were transfected with pEZ-LV206-PANDAR, pEZ-LV206-control and extracted with Plasmid Midiprep System, Promega. PC9 and A549 cells were cultured for 24 h and transfected into six-well plate using Lipofectamine3000. The transfected cell lines were harvested after 48 h or 72 h for follow-up series of cell assays. Besides, the experimental methods of two plasmids co-transfection were same as above.
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2

TcC Variant Mutagenesis and Expression

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All DNA manipulations described in this work were performed by standard methods.41 Megaprimers for the TcC variants, possessing the specific mutation (Table S1) were purchased from Microsynth AG (Switzerland) and used in two-stage PCR reactions to introduce mutations using Pfu DNA polymerase (Promega GmbH, Germany) and pET26b(+)_TcC as template. Restriction enzymes were purchased from New England Biolabs and used following the manufacturer's protocol. Plasmid DNA was prepared using PureYield™ Plasmid Miniprep and Plasmid Midiprep system (Promega GmbH, Germany). The amplified products were chemically transformed in Escherichia coli XL-10.
For HiC the codon-optimized genes (i.e. E. coli codon usage) of wild-type, I167Q and L64H/I167Q variants were purchased from GeneArt® (Life Technologies, USA). The genes were cloned into the vector pET26b(+) and chemically transformed by heat shock into E. coli XL-10.
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