Human leptin picokine elisa kit

Levels of adipokines (leptin, adiponectin, and Interleukin-6 (IL-6)) were investigated on D10 of differentiation in a medium collected from mature adipocytes using commercial kits: Human IL-6 PicoKine™ ELISA Kit (Boster, San Mateo, CA, USA), Human Leptin PicoKine™ ELISA Kit (Boster, San Mateo, CA, USA), and a Human ADIPOQ/Adiponectin ELISA Kit (Sigma-Aldrich, St. Louis, USA).

The diagnosis of T2DM was determined by the use of oral hypoglycemic agents and/or fasting glucose (≥126 mg/dL) and/or glycosylated hemoglobin (≥6.5%) (1 (link)). The following markers were also analyzed: fasting insulin, homeostasis model assessment of insulin resistance index (HOMA-IR index), and C-reactive protein. Blood was collected after 12 hours of fasting. HOMA-IR result was defined by: HOMA-IR = fasting serum insulin (U/mL) × fasting plasma glucose (mmol/L)/22.5.
Interleukins (IL)-4), IL-10, monocyte chemoattractant protein 1 (MCP-1), and TNFα were examined using the Bio-Plex^® Pro Human Cytokine 27-Plex kit (Bio-Rad, Hercules, CA) per manufacturer’s instructions in the Bio-Plex 200 System provided by the Oswaldo Cruz Foundation, Rio de Janeiro. Enzyme-linked immunosorbent assays were used to measure adiponectin (Human Adiponectin PicoKine™ ELISA Kit, Boster Bio, Pleasanton, CA), leptin (Human Leptin PicoKine™ ELISA Kit, Boster Bio, Pleasanton, CA), IL-1β, and IL-6 (Human IL-1β PicoKine™ ELISA Kit and Human IL-6 PicoKine™ ELISA Kit, Boster Bio, Pleasanton, CA, respectively) per manufacturer’s instructions. All measurements were performed at the Laboratory of Immunopathology of Infectious Diseases.