Eight-week-old Sprague–Dawley male rats (180–200 g) were divided into three groups (six rats/group) and all were given chow mixed with 5% HLP (weight/weight HLP/chow) for 8 weeks. After 4 weeks administration of HLP, rats were injected with ASC-J9® (37.5 mg/kg/48 h i.p.) or control vehicle DMA, and AgomiR-185-5p or AgomiR-negative control (15 nmol/kg/week) for 4 weeks. The AgomiRs were synthesized by Ribobio Inc. (Guangzhou, China). Next, the rats were sacrificed, and kidneys were obtained for analyses.
Agomirs
Manufactured by RiboBio
Sourced in China
AgomiRs are small, chemically modified RNA molecules designed to mimic the structure and function of naturally occurring microRNAs (miRNAs). They are used in research applications to modulate the expression of specific genes by targeting and binding to complementary messenger RNA (mRNA) molecules, thereby influencing cellular processes.
Automatically generated - may contain errors
4 protocols using agomirs
1
Evaluating ASC-J9 and AgomiR-185-5p in HLP-Induced Kidney Injury
2
Transfection of miRNA mimics, agomirs, and inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MiRNA mimics(50 nmol/L), agomirs, and inhibitors (100 nmol/L)(RiboBio, Guangzhou, China) were transfected into cells using Lipofectamine 2000 (Invitrogen, Carlsbad, USA). At forty-eight hours post-transfection, cells were harvested for further analyses.
3
MiRNA Modulation and ING5 Silencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mimic, agomiRs, antagomiRs, siRNAs, the scrambled sequence (negative control, NC) and the riboFECT CP transfection kit were supplied by Ribobio (Guangzhou, China). The GFP-tagged overexpression ING5 construct (construct from pReciever-M98) was purchased from Genecopia, (Guangzhou, China). Transfection of both the ribonucleic acid reagents mentioned above and the reporter plasmids was performed according to the manufacturer's instructions.
Chemically modified mimic oligonucleotides (agomiRs) were synthesized to regulate miR-193a-3p/5p expression in vivo. The 3′ end of the oligonucleotides was conjugated to cholesterol, and all of the bases were 2′-OMe modified. The agomiR oligonucleotides were deprotected, desalted and purified by high-performance liquid chromatography.
The siRNA sequences used for ING5 interference in this study were as follows:
si-ING5-1:
5′ CCAUGUACUUGGAGCACUAdTdT 3′
3′ dTdTGGUACAUGAACCUCGUGAU 5′
si-ING5-2:
5′ GGAAUACAGUGACGACAAAdTdT 3′
3′ dTdTCCUUAUGUCACUGCUGUUU 5′
si-ING5-3:
5′ CCUACGAGAUGGUGGAUAAdTdT 3′
3′ dTdTGGAUGCUCUACCACCUAUU 5′
Chemically modified mimic oligonucleotides (agomiRs) were synthesized to regulate miR-193a-3p/5p expression in vivo. The 3′ end of the oligonucleotides was conjugated to cholesterol, and all of the bases were 2′-OMe modified. The agomiR oligonucleotides were deprotected, desalted and purified by high-performance liquid chromatography.
The siRNA sequences used for ING5 interference in this study were as follows:
si-ING5-1:
5′ CCAUGUACUUGGAGCACUAdTdT 3′
3′ dTdTGGUACAUGAACCUCGUGAU 5′
si-ING5-2:
5′ GGAAUACAGUGACGACAAAdTdT 3′
3′ dTdTCCUUAUGUCACUGCUGUUU 5′
si-ING5-3:
5′ CCUACGAGAUGGUGGAUAAdTdT 3′
3′ dTdTGGAUGCUCUACCACCUAUU 5′
4
Modulating miR-320b in MRL/lpr Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fourteen female MRL/lpr mice were purchased from SLRC Experimental Animals Co., Ltd. (Shanghai, China) and maintained in a controlled environment (20 ± 2°C, 12-hr/12-hr light/dark cycle) under specific pathogen-free conditions at the Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University. All animal studies were approved by Animal Care and Use Committee of the hospital. All procedures were performed in accordance with the guidelines of the hospital.
Sixteen-week-old MRL/lpr mice were divided into NC (n = 5), miR-320b agomir (n = 4), and miR-320b antagomir (n = 5) groups. A total of 20 nmol miR-320b Agomirs, antAgomirs, or NC were injected intravenously every 6 days for four times. All mice were sacrificed 19 days after the first administration. Agomirs, antAgomirs, and NC were purchased from RiboBio Co., Ltd. (Guangzhou, China). Urine and blood samples were collected every week until the end of the experiment. The concentrations of urinary protein were measured using Bradford protein quantitation assay (KeyGen, Nanjing, China). Cytokine concentrations were measured by ELISA (MultiSciences Biotech, Nanjing, China) according to the manufacturer's instructions.
Sixteen-week-old MRL/lpr mice were divided into NC (n = 5), miR-320b agomir (n = 4), and miR-320b antagomir (n = 5) groups. A total of 20 nmol miR-320b Agomirs, antAgomirs, or NC were injected intravenously every 6 days for four times. All mice were sacrificed 19 days after the first administration. Agomirs, antAgomirs, and NC were purchased from RiboBio Co., Ltd. (Guangzhou, China). Urine and blood samples were collected every week until the end of the experiment. The concentrations of urinary protein were measured using Bradford protein quantitation assay (KeyGen, Nanjing, China). Cytokine concentrations were measured by ELISA (MultiSciences Biotech, Nanjing, China) according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!