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Kinase selectivity profiling assay kit

Manufactured by Promega
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The Kinase Selectivity Profiling Assay Kit is a laboratory tool used to evaluate the selectivity of a compound against a panel of kinases. The kit provides a convenient method to screen compound activity across multiple kinases in a single experiment.

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Lab products found in correlation

Luteolin, by Merck Group (2 mentions) Staurosporine, by Merck Group (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) Tdzd 8, by Merck Group (2 mentions) β amyloid fragment peptide 1 42 aβ42, by AnaSpec (2 mentions) Celltiter 96 aqueous one solution cell proliferation mtx assay kit, by Promega (2 mentions) Adp glo kinase assay kit, by Promega (2 mentions) Tau ps396 human elisa kit, by Thermo Fisher Scientific (1 mentions) Cell extraction buffer, by Thermo Fisher Scientific (1 mentions) Desipramine, by Merck Group (1 mentions) Orientin, by Merck Group (1 mentions) Theophylline, by Merck Group (1 mentions) Atenolol, by Merck Group (1 mentions) Isovitexin, by Merck Group (1 mentions) Isoorientin, by Merck Group (1 mentions) Tmschn2, by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Gsk 3β, by Thermo Fisher Scientific (1 mentions) Gsk3β ps9, by Thermo Fisher Scientific (1 mentions) Tau 5, by Thermo Fisher Scientific (1 mentions)

2 protocols using kinase selectivity profiling assay kit

1

Evaluating Natural Flavones and Kinase Inhibitors

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All solvents and reagents were from commercial sources and were used without further purification. Natural flavones (isoorientin, orientin, isovitexin, and luteolin), staurosporine, TDZD-8, theophylline, atenolol, desipramine, TMSCHN2, [TEMPO]+[BF4]−, HCTU, organic amines, and protease inhibitor cocktail were from Sigma-Aldrich (Saint Louis, MO). β-Amyloid fragment peptide 1–42 (Aβ42) was from AnaSpec (Fremont, CA). Kinase Selectivity Profiling Assay Kit, ADP-Glo Kinase Assay Kit, and CellTiter 96 AQueous One Solution Cell Proliferation MTX Assay Kit were from Promega (Madison, WI). Human Tau pS396 ELISA Kit and Cell Extraction Buffer were from Invitrogen (Camarillo, CA). Precoated PAMPA plate system was from Corning (Tewksbury, MA).
Liang Z, & Li Q.X. (2018). Discovery of Selective, Substrate-Competitive, and Passive Membrane Permeable Glycogen Synthase Kinase-3β Inhibitors: Synthesis, Biological Evaluation, and Molecular Modeling of New C-Glycosylflavones. ACS chemical neuroscience, 9(5), 1166-1183.
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2

Alzheimer's Disease In Vitro Assays

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Staurosporine, 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione (TDZD-8), lithium chloride (LiCl), luteolin, Triton X-100, protease inhibitor cocktail, and 5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium (BCIP/NBT) reagents were from Sigma-Aldrich (Saint Louis, MO, USA). β-Amyloid fragment peptide 1–42 (Aβ42) was from AnaSpec (Fremont, CA, USA). Kinase Selectivity Profiling Assay Kit, ADP-Glo Kinase Assay Kit, and CellTiter 96 AQueous One Solution Cell Proliferation MTX Assay Kit were from Promega (Madison, WI, USA). Antibodies Tau-5, Tau pS199/pS202, Tau pS396, GSK3β, and GSK3β pS9 were from Invitrogen (Camarillo, CA, USA). β-Actin antibody was from GenScript (Piscataway, NJ, USA). Alkaline phosphatase-conjugated secondary antibodies were from Southern Biotech (Birmingham, AL, USA).
Liang Z., Zhang B., Su W.W., Williams P.G, & Li Q.X. (2016). C-Glycosylflavones Alleviate Tau Phosphorylation and Amyloid Neurotoxicity through GSK3β Inhibition. ACS chemical neuroscience, 7(7), 912-923.
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