The following antibodies and dilution were used for staining: rabbit anti-GFP (Medical & Biological Laboratories Co., Ltd, #598, 500 × ), anti-beta-galactosidase (MP Biomedicals, #0855976, 1000 × ), rat anti-DE-cad (DCAD2, 20 × )61 (link), mouse anti-Gasp (2A12, DSHB, 2 × ), anti-Serp (300 × ) and Verm (300 × )34 (link), anti-Pio (50 × )36 (link), anti-Arl3 (500 × )19 (link) and CBP-Alexa 488 (50 × )35 (link). Embryos were dechorionated with 1/2 diluted commercial bleach and were fixed by mixing vigorously in the mixture of 2 ml 4% paraformaldehyde in PBS and 2 ml heptane in liquid scintillation vial for 30 min at room temperature. After removal of heptane, embryos were devitelinized by addition of 2 ml methanol and vigorous mixing. Devitellinized embryos were washed two times with methanol and rehydrated with PBS. Embryos were stained as described previously56 (link). Blocking, antibody incubation and washing were performed with 1% BSA and 0.2% Triton X-100 in PBS. The anti-Katanin 60 antibody (100 × ) was generously donated by Naoyuki Fuse and Fumio Matsuzaki.
Rabbit anti gfp
Manufactured by Medical & Biological Laboratories
Rabbit anti-GFP is a primary antibody that specifically recognizes and binds to the Green Fluorescent Protein (GFP). It is a powerful tool used in various research applications to detect and visualize the expression of GFP-tagged proteins in cells and tissues.
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2 protocols using rabbit anti gfp
1
Immunostaining Drosophila Embryos
2
Immunostaining Drosophila Abdominal Cuticles
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Immunostaining of Drosophila abdominal cuticles was performed as described (Yasunaga et al. 2010 (link)). Briefly, abdominal cuticles were dissected and fixed in 4% paraformaldehyde in PBS for 30–60 min at room temperature followed by blocking for 60 min in PBS containing 0.3% Triton X-100 and 5% normal goat serum. Tissues were stained with mouse anti-GFP (1:1000; 3F6, Wako), rabbit anti-GFP (1:500; polyclonal, Medical and Biological Laboratories), and/or rat anti-CD8a (1:100; Caltag). Images were taken on a Leica TCS SP8 confocal microscope (Leica) and adjusted for brightness and contrast with Adobe Photoshop (Adobe Systems).
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