Small interfering RNA experiments were performed by using human-specific NRF2 or non-targeting siRNA (On-TargetPlus SMART pool human NFE2L2; Dharmacon). Cells were transfected with 50 nM siRNA, using Dharmafect reagent (Thermo Scientific), according to the manufacturer's instructions.
On targetplus smart pool human nfe2l2
Manufactured by Horizon Discovery
The On-TargetPlus SMART pool human NFE2L2 is a gene-specific siRNA pool designed to target the human NFE2L2 (Nuclear Factor, Erythroid 2 Like 2) gene. The pool consists of a mixture of multiple siRNA duplexes that work together to effectively silence the target gene expression.
Automatically generated - may contain errors
2 protocols using on targetplus smart pool human nfe2l2
1
NRF2 Gene Silencing Protocol
2
MCF7-AREc32 Cell Transfection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1.5×106 MCF7-AREc32 cells were reverse transfected in 100-mm Tissue Culture dishes with 40 µl LipofectAMINE RNAiMAX (Invitrogen) and 250 pmoles of siRNA. The siRNAs that were used were ON-TARGETplus Non-targeting siRNA #4 (Dharmacon) and ON-TARGET plus SMARTpool human NFE2L2 (Dharmacon).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!