L cysteine l cys

Minimum essential medium (MEM), fetal bovine serum (FBS), penicillin/streptomycin, Hanks Balanced Salt Solution, sodium pyruvate and trypsin were purchased from Gibco (Carlsbad, CA, United States). 5-Alpha-dihydrotestosterone (5α-DHT), collagenase IA, pyridoxal 5-phosphate, O-(carboxymethyl) hydroxylamine hemihydrochloride chloride (AOAA), DL-propargylglycine (PAG), sodium hydrosulfide hydrate (NaHS × H₂O), sodium lactate, L-cysteine (L-Cys), bumetanide, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), glibenclamide (Glib), Iberiotoxin (IbTx) and tetraethylammonium chloride (TEA) were purchased from Sigma-Aldrich (St. Louis, MO, United States). HC067047 was purchased from Tocris (Bristol, United Kingdom). NaCl, KCl, MgSO₄, FeCl₃, HCl, BaCl₂, NaHCO₃, KH₂PO₄, CaCl₂, glucose, mannitol, lactic acid, normal saline, N,N-dimethyl-p-phenylenediamine sulfate, trichloroacetic acid, and zinc acetate were purchased from Guangzhou Chemical Pharmaceutical Factory (Guangzhou, China). Universal two-step detection kit (PV-9000) and DAB detection kit were purchased from ZSBIO (Beijing, China). Fluo-3 AM was purchased from Molecular Probes (Eugene, OR, United States). PBFI AM was purchased from Cayman Chemical (Ann Arbor, MI, United States).

L-Cysteine (L-Cys), aminooxyacetic acid (AOAA, CBS inhibitor), DL-propargylglycine (PAG, CSE inhibitor), lipopolysaccharide (LPS), and MCC950 (NLRP3 inflammasome inhibitor) were purchased from Sigma-Aldrich (St. Louis, MO). Control liposomes and clodronate liposomes were purchased from FormuMax (Silicon Valley, CA). Deoxyribonuclease Ⅰ was purchased from Beijing Solarbio Science and Technology Co. (Beijing, China). 2-Deoxy-D-glucose was purchased from J&K Scientific (Beijing, China). Armenian Hamster IgG Isotype Control and IL-1 beta Monoclonal Antibody were purchased from Invitrogen Life Technology (Foster City, CA). Antibodies for βⅢ-tubulin, Nestin, and IL-1β were purchased from Abcam (Cambridge, United Kingdom). Antibodies for cleaved caspase-1 and NLRP3 were purchased from Cell Signaling Technology (Danvers, MA). Monoclonal mouse anti-β-actin was purchased from Zhongshan Golden Bridge Biotechnology (Beijing, China). The secondary antibodies were purchased from Abcam (Cambridge, United Kingdom), Invitrogen Life Technology (Foster City, CA), and Zhongshan Golden Bridge Biotechnology (Beijing, China). Mouse ELISA kits were purchased from Dakewe Biotech (Shenzhen, China) and Boster Biological Technology (Wuhan, China). Dulbecco’s modified Eagle’s medium (DMEM), 10% heat-inactivated FBS, and 1% penicillin–streptomycin solutions were purchased from Gibco (Foster City, CA).

Yttrium(III) acetate hydrate (99.9%), ytterbium(III) acetate hydrate (99.9%), erbium(III) acetate hydrate (99.9%), neodymium(III) acetate hydrate (99.9%), ammonium fluoride (NH₄F, 98+%), 1-octadecene (ODE, 90%), oleic acid (OA, 90%), epirubicin hydrochloride (EPI, 90+%), and L-cysteine (L-Cys, 97%) were purchased from Sigma-Aldrich. Histidine (His, 99%) and glucose (Glu, 99%) were bought from Macklin. Phosphate buffered saline (PBS) was obtained from HyClone. Hydrogen chloride (HCl), sodium hydroxide (NaOH), potassium chloride (KCl), sodium chlorate (NaCl), calcium chloride (CaCl₂,) magnesium chloride (MgCl₂), aluminum chloride (AlCl₃), and L-glycine (L-Gly) were supplied from the China National Pharmaceutical Group Corporation. Cyanidin 3-glucoside, doxorubicin, and daunorubicin were purchased from Shanghai yuanye Bio-Technology company. All chemicals and reagents were analytically pure and used without any further purification.