For apoptosis analysis, transfected cells were collected by trypsin 48 h after transfection and then stained with Annexin V-FITC and PI using Cell apoptosis Analysis Kit (Beyotime Biotechnology, China). Cell apoptosis was analyzed by flow cytometry (FACScan; BD Biosciences) using CellQuest software (BD Biosciences).
Cell apoptosis analysis kit
Manufactured by Beyotime
Sourced in China
The Cell Apoptosis Analysis Kit is a laboratory equipment designed to detect and quantify apoptosis, a form of programmed cell death, in cell samples. The kit provides the necessary reagents and protocols for the analysis of apoptotic cells using various techniques, such as flow cytometry or fluorescence microscopy. This product enables researchers to study the mechanisms and dynamics of cell apoptosis for a wide range of applications in the life sciences and biomedical fields.
Automatically generated - may contain errors
Lab products found in correlation
Facscan, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
Cytoflex flow cytometer, by Beckman Coulter (1 mentions)
Attune nxt flow cytometer, by Thermo Fisher Scientific (1 mentions)
Cell cycle analysis kit, by Beyotime (1 mentions)
Byflow cytometer facscalibur, by BD (1 mentions)
Guava easycyte 6ht 2l, by Merck Group (1 mentions)
Flow cytometry, by BD (1 mentions)
7 protocols using cell apoptosis analysis kit
1
Apoptosis Analysis by Flow Cytometry
2
Apoptosis Analysis of HCT116 and LOVO Cells
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The apoptosis of HCT116 and LOVO cells was determined using Cell Apoptosis Analysis Kit (C1062, Beyotime, China). Transfected or/and irradiated cells (1 × 105 (link)/well) were stained with 5 μL of Annexin V-FITC and 10 μL of propidium iodide solution at room temperature for 10 min in the dark. Lastly, cell apoptotic rate was quantified using a CytoFLEX flow cytometer (Beckman Coulter, USA).
3
Cell Cycle Analysis of HUVEC Treated with PRP-Derived Extracellular Vesicles
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Flow cytometry was used to analyse HUVEC cell cycle treated with PRP-Exos, PRP-AS and NC. All steps were performed according to the instructions of the cell cycle and cell apoptosis analysis kit (C1052, Beyotime). Measurement of the cell cycle distribution was performed using a flow cytometer (Beckman, USA).
4
Apoptosis Quantification in A375 and C32 Cells
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Briefly, A375 and C32 cells were washed twice with ice cold PBS, and 1× binding buffer was used to make a 1×106 cell/mL suspension. The cells were then mixed with a cell apoptosis analysis kit (Beyotime, Shanghai, China) at room temperature for 15 min under dark conditions, according to the manufacturer’s instructions. Finally, red fluorescence was detected at a 488 nm wavelength, as well as light scattering using a Attune NxT flow cytometer (Thermo Fisher, USA).
5
Cell Cycle and Apoptosis Analysis
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After transfected with siRNA for 48 h, cells were harvested by trypsin, and then
cells were fixed with 70% ethanol at 4°C overnight. Then fixed
cells were incubated with RNase A for 30 min to completely degrade RNA, and then
stained with propidium oxide for another 30 min in dark place using Cell Cycle
Analysis Kit (Beyotime Biotechnology, China). The managed cells were detected by
flow cytometer FACSCalibur (BD Bioscience, U.S.A.) and analyzed with Flowjo
software.
For apoptosis analysis, transfected cells were collected by trypsin 48 h after
transfection, and then stained with Annexin V-FITC and PI using Cell apoptosis
Analysis Kit (Beyotime Biotechnology, China). Stained cells were detected by
flow cytometer FACSCalibur (BD Bioscience, U.S.A.) and analyzed with Flowjo
software.
cells were fixed with 70% ethanol at 4°C overnight. Then fixed
cells were incubated with RNase A for 30 min to completely degrade RNA, and then
stained with propidium oxide for another 30 min in dark place using Cell Cycle
Analysis Kit (Beyotime Biotechnology, China). The managed cells were detected by
flow cytometer FACSCalibur (BD Bioscience, U.S.A.) and analyzed with Flowjo
software.
For apoptosis analysis, transfected cells were collected by trypsin 48 h after
transfection, and then stained with Annexin V-FITC and PI using Cell apoptosis
Analysis Kit (Beyotime Biotechnology, China). Stained cells were detected by
flow cytometer FACSCalibur (BD Bioscience, U.S.A.) and analyzed with Flowjo
software.
6
Cell Cycle and Apoptosis Analysis
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The cells were seeded into 6-well plates and grown until > 80% confluence. The cells were then treated with CA, TMZ, or CA + TMZ at the indicated concentrations for 24 h. For cell cycle analysis, cells were trypsinized and fixed in 70% ethanol overnight. The cells were stained according to the manufacturer’s protocol using the cell cycle analysis kit (Beyotime, Shanghai, China) and detected by flow cytometry (Guava EasyCyte 6HT-2L, Merck Millipore, Darmstadt, Germany). For cell apoptosis analysis, the cells were stained with the cell apoptosis analysis kit (Beyotime, Shanghai, China) according to the manufacturer’s protocol and detected by flow cytometry (Guava EasyCyte 6HT-2L, Merck Millipore, Darmstadt, Germany).
7
Apoptosis Assay for Liver Cancer Cells
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Huh-7 and HCC-LM3 cells were resuspended and washed with PBS for 3 times, and cell were stained with Cell Apoptosis Analysis Kit (Beyotime, China) based on the (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted March 24, 2021. ; https://doi.org/10.1101/2021.03.24.436699 doi: bioRxiv preprint manufacturer's instructions. Flow cytometry (BD, USA) was used to analyze the staining and the data were analyzed with FlowJo 7.6 software (USA).
The copyright holder for this preprint this version posted March 24, 2021. ; https://doi.org/10.1101/2021.03.24.436699 doi: bioRxiv preprint manufacturer's instructions. Flow cytometry (BD, USA) was used to analyze the staining and the data were analyzed with FlowJo 7.6 software (USA).
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