Anti hoxd10

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-HOXD10 is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to the HOXD10 protein, which is a member of the homeobox family of transcription factors. This product can be utilized in various experimental techniques to study the expression, localization, and interactions of the HOXD10 protein.

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Lab products found in correlation

Anti actin hrp antibody, by Santa Cruz Biotechnology (2 mentions) Anti bim, by Cell Signaling Technology (2 mentions) Dnase, by Roche (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Ivis spectrum imaging station, by PerkinElmer (1 mentions) Enhanced chemi luminescence (ecl), by Roche (1 mentions) Anti vimentin, by Proteintech (1 mentions) Anti apc2, by Abcam (1 mentions) Anti zeb1, by Santa Cruz Biotechnology (1 mentions) Anti snail, by Abcam (1 mentions) Anti gapdh, by Proteintech (1 mentions) Dab substrate, by Roche (1 mentions) Ripa buffer, by Roche (1 mentions) Anti pten, by Santa Cruz Biotechnology (1 mentions) Anti rabbit igg hrp, by New England Biolabs (1 mentions) Anti mouse igg hrp, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-HOXD10 (H-80, (2 citations)

4 protocols using anti hoxd10

Immunoblot Analysis of BIM and HOXD10

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Cells treated with various drug combinations were washed with cold PBS and lysed in RIPA buffer containing 1mg ml⁻¹ DNAse (Roche Life Science, Indianapolis, IN) and supplemented with protease inhibitor cocktail (Roche Life Science, Indianapolis, IN). Cell lysates, stored frozen at −80 °C were resolved on 4–20% SDS-PAGE gels and transferred onto a nitrocellulose membrane. The membranes were first stained with anti-BIM (Cell Signaling Technology, Danvers, MA), and anti-HOXD10 (Santa Cruz Biotechnology, Dallas, TX) antibody cocktail followed by peroxidase conjugated-anti-rabbit antibody. ECL (Roche Life Science, Indianapolis, IN) was used to develop blots. Signal intensity was recorded on an IVIS Spectrum imaging station (Perkin Elmer, Hopkinton, MA). After documenting signal, the blot was subjected to a second round of immunostaining using an anti-actin-HRP antibody (Santa Cruz Biotechnology, Dallas, TX).

Yoo B., Kavishwar A., Ross A., Wang P., Tabassum D.P., Polyak K., Barteneva N., Petkova V., Pantazopoulos P., Tena A., Moore A, & Medarova Z. (2015). Combining miR10b-targeted nanotherapy with low-dose doxorubicin elicits durable regressions of metastatic breast cancer. Cancer research, 75(20), 4407-4415.

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Western Blotting and DNA Electrophoresis

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Western blotting was performed as previously described 18 (link). The primary antibodies used were anti-EZH2, anti-β-catenin, anti-P-β-catenin (Ser675), anti-H3 (1:1000 CST), anti-PCDHB5, anti-PCDH10, anti-Vimentin, anti-GAPDH (1:500 Proteintech), anti-HOXD10, anti-ZEB1 (1:300 SANTA CRUZ), anti-APC2, and anti-Snail (1:1000 Abcam). For DNA electrophoresis, 10 ul of sample DNA was loaded into each well of a 2% agarose gel and electrophoresed at 80 V for half an hour. Subsequently, the gel was imaged by ultraviolet light.

Li Y., Ren Y., Wang Y., Tan Y., Wang Q., Cai J., Zhou J., Yang C., Zhao K., Yi K., Jin W., Wang L., Liu M., Yang J., Li M, & Kang C. (2019). A Compound AC1Q3QWB Selectively Disrupts HOTAIR-Mediated Recruitment of PRC2 and Enhances Cancer Therapy of DZNep. Theranostics, 9(16), 4608-4623.

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Immunoblot Analysis of Signaling Proteins

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Cells treated with various drug combinations were washed with cold PBS and lysed in RIPA buffer containing 1 mg/mL DNAse (Roche Life Science) and supplemented with protease inhibitor cocktail (Roche Life Science). Cell lysates stored frozen at −80°C, were resolved on 4%–20% SDS-PAGE gels and transferred onto a nitrocellulose membrane. The membranes were first stained with anti-BIM (Cell Signaling Technology), anti-HOXD10 (Santa Cruz Biotechnology) or anti-PTEN (Santa Cruz Biotechnology) antibody followed by peroxidase-conjugated anti-rabbit antibody. DAB substrate (Roche Life Science) was used to develop blots. After documenting signal, the blot was subjected to a second round of immunostaining using an anti-actin HRP antibody (Santa Cruz Biotechnology).

Chen M., Kim B., Robertson N., Mondal S.K., Medarova Z, & Moore A. (2023). Co-administration of temozolomide (TMZ) and the experimental therapeutic targeting miR-10b, profoundly affects the tumorigenic phenotype of human glioblastoma cells. Frontiers in Molecular Biosciences, 10, 1179343.

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Immunoblotting with HOXD10 and AMOT-p80 Antibodies

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Antibodies used were goat polyclonal anti-HOXD10 (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-HOXD10, rabbit polyclonal anti-AMOT-p80 (both Abcam, Cambridge, UK) and mouse monoclonal anti-β-actin (Sigma Aldrich), anti-goat IgG horseradish peroxidase (HRP; Amersham Ltd, Amersham, UK), anti-rabbit IgG HRP (New England Biolabs, Hitchin, UK) and anti-mouse IgG HRP (Cell Signalling, Hitchin, UK).

Hakami F., Darda L., Stafford P., Woll P., Lambert D.W, & Hunter K.D. (2014). The roles of HOXD10 in the development and progression of head and neck squamous cell carcinoma (HNSCC). British Journal of Cancer, 111(4), 807-816.

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