Secondary antibodies conjugated with horseradish peroxidase hrp

Analytical grade cis-bixin (9-cis-6,6′-diapo-ψ,ψ-carotenedioic acid, 6-methyl ester) was purchased from Spectrum (CAS number: 6983-79-5). LC/MS confirmation of purity (>98% by weight) was performed using electrospray mass spectrometry of bixin [dissolved in tetrahydrofuran and diluted 10-fold in acetonitrile/NH₄OH (0.1 N); ESI-MS (negative ion mode) m/z 393.21 (M - 1)^-] employing a Bruker Apex FT/ICR mass spectrometer, as specified before (Tao et al., 2015 (link)). Polyethylene glycol 400 (PEG400) was from EMD Millipore. Xanthotoxin (8-methoxypsoralen, 8-MOP), and hydrogen peroxide (H₂O₂) were from Sigma. Primary antibodies against NRF2, KEAP1, TRXR1, GCLM, NQO1, HO1, OGG1, GAPDH, and actin were purchased from Santa Cruz Biotechnology. Primary antibody against p62 was from Abnova. Secondary antibodies conjugated with horseradish peroxidase (HRP) were purchased from Sigma. Antibody against 8-oxo-deoxyguanosine (8-oxo-dG) was from Trevigen.

The anti-eIF4G, anti-ribosomal protein S5 (RPS5), anti-RPLP0, anti-eIF2a, anti-eIF3A, anti-eIF3e, anti-eIF4A, anti-eIF5B, and anti-RALY monoclonal antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-FLAG monoclonal antibody was purchased from Proteintech (Chicago, IL, USA). Anti-β-actin monoclonal antibody was purchased from Kangwei Century (Jiangsu, China). Anti-HA monoclonal antibody, the protein synthesis inhibitor cycloheximide (CHX), and secondary antibodies conjugated with horseradish peroxidase (HRP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Polyclonal pig antiserum directed against FMDV and polyclonal rabbit antiserum against SVA (Senecavirus A) and EMCV (Encephalomyocarditis virus) were generated and stored in our laboratory. Transfection reagents Lipofectamine LTX for transfection recombinant plasmids and Lipofectamine RNAiMax Reagent for small interfering RNA (siRNA) transfection were purchased from Invitrogen (CA, USA). The RNA extraction reagent TRIzol was purchased from Invitrogen.