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Anti trps1

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Anti-TRPS1 is a laboratory reagent designed for use in research applications. It is a specific antibody that recognizes the TRPS1 protein, which is involved in various cellular processes. The core function of Anti-TRPS1 is to enable the detection and analysis of TRPS1 expression in biological samples.

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Lab products found in correlation

Anti znf217, by Santa Cruz Biotechnology (2 mentions) Truseq chip sample prep kit, by Illumina (2 mentions) Anti er, by Santa Cruz Biotechnology (2 mentions) Anti lsd1, by Abcam (2 mentions) Anti h3k27ac, by Abcam (2 mentions) Anti flag, by Merck Group (1 mentions) Anti actin, by Proteintech (1 mentions) Anti h4, by Merck Group (1 mentions) Anti hdac2, by Cell Signaling Technology (1 mentions) Anti h4k16ac, by Merck Group (1 mentions) Anti gal4, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-TRPS1 antibody (2 citations)

4 protocols using anti trps1

1

Antibody Sources and Applications

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Antibodies used in this study and their sources are as follows: anti-TRPS1 (R&D Systems#AF4838), anti-HDAC2 (Cell Signaling Technology#5113), anti-H4K16ac (Millipore#39929), anti-H4 (Millipore#04–858), anti-USP4 (Cell Signaling Technology#2651), anti-actin (proteintech#60008–1-Ig), anti-HA (Biotool#B23402), anti-Flag (Sigma#F7425), anti-Myc (Biotool#B23402), anti-His (Cell Signaling Technology#12698), and anti-Gal4 (Santa Cruz#510).
Wang Y., Zhang J., Wu L., Liu W., Wei G., Gong X., Liu Y., Ma Z., Ma F., Thiery J.P, & Chen L. (2018). Tricho-rhino-phalangeal syndrome 1 protein functions as a scaffold required for ubiquitin-specific protease 4-directed histone deacetylase 2 de-ubiquitination and tumor growth. Breast Cancer Research : BCR, 20, 83.
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2

ChIP-seq Protocol with Antibody Details

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The antibodies used for the ChIP-seq were anti-coREST (Abcam, ab32631), anti-ER (Santa-Cruz, ref. sc-543), anti-H3K27ac (Abcam, ref. ab4729), anti-LSD1 (Abcam, ab17721), anti-TRPS1 (R&D Systems, ref. AF4838), anti-ZNF217 (Santa-Cruz, sc-55351) and rabbit serum (sc-2027). ChIP were performed as previously described in Schmidt et al. (Methods, 2010), using 10 ug of antibody and 60 million cells. The ChIP-seq and the input libraries were prepared using the TruSeq ChIP Sample Prep Kit (Illumina, ref. IP-202-1012).
Serandour A.A., Mohammed H., Miremadi A., Mulder K.W, & Carroll J.S. (2018). TRPS1 regulates estrogen-receptor binding and histone acetylation at enhancers. Oncogene, 37(39), 5281-5291.
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3

ChIP-seq Protocol with Antibody Details

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The antibodies used for the ChIP-seq were anti-coREST (Abcam, ab32631), anti-ER (Santa-Cruz, ref. sc-543), anti-H3K27ac (Abcam, ref. ab4729), anti-LSD1 (Abcam, ab17721), anti-TRPS1 (R&D Systems, ref. AF4838), anti-ZNF217 (Santa-Cruz, sc-55351) and rabbit serum (sc-2027). ChIP were performed as previously described in Schmidt et al. (Methods, 2010), using 10 ug of antibody and 60 million cells. The ChIP-seq and the input libraries were prepared using the TruSeq ChIP Sample Prep Kit (Illumina, ref. IP-202-1012).
Serandour A.A., Mohammed H., Miremadi A., Mulder K.W, & Carroll J.S. (2018). TRPS1 regulates estrogen-receptor binding and histone acetylation at enhancers. Oncogene, 37(39), 5281-5291.
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4

Immunoprecipitation and Mass Spectrometry Analysis

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Protein lysates were prepared using RIPA lysis buffer (10 mM Tris-HCl at pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.1% SDS, 1% Triton X-100, 1% deoxycholate in milli-Q) supplemented with protease inhibitors. The whole-cell lysates were incubated with 100 μL of Protein A or G magnetic beads (ThermoFisher Scientific 10002D/10004D) prebound with anti-TRPS1 (R&D Systems), anti-GATA3, normal goat IgG control, or normal mouse IgG control (Supplemental Table S3). The immunoprecipitates were analyzed using Western blot after elution in sample buffer at 95°C or using LC/MS-MS analysis (Supplemental Material).
Cornelissen L.M., Drenth A.P., van der Burg E., de Bruijn R., Pritchard C.E., Huijbers I.J., Zwart W, & Jonkers J. (2020). TRPS1 acts as a context-dependent regulator of mammary epithelial cell growth/differentiation and breast cancer development. Genes & Development, 34(3-4), 179-193.
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