Log In Sign up
The largest database of trusted experimental protocols

Pierce hela tryptic digest standard

Manufactured by Thermo Fisher Scientific
Visit Supplier

The Pierce HeLa tryptic Digest Standard is a reference material for mass spectrometry-based proteomics analysis. It is a tryptic digest of HeLa cell lysate, providing a complex mixture of peptides derived from a variety of human cellular proteins. This standard can be used for method development, quality control, and performance evaluation of mass spectrometry instrumentation and data analysis workflows.

Automatically generated - may contain errors

Lab products found in correlation

Rapigest sf, by Waters Corporation (1 mentions) Qubit fluorimetric protein assay, by Thermo Fisher Scientific (1 mentions) Hplc grade water, by Thermo Fisher Scientific (1 mentions) Ltq orbitrap xl etd, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Formamide, by Merck Group (1 mentions) Magic c18aq, by Bruker (1 mentions)

Spelling variants of this product

HeLa digest (4 citations) HeLa digest standard (3 citations) HeLa tryptic digest standard (2 citations)

2 protocols using pierce hela tryptic digest standard

1

Protein Extraction and Digestion Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were precipitated using methanol/chloroform and resuspended in 100 μL of RapiGest SF (Waters). Total protein concentration was measured using the Qubit fluorimetric protein assay (Thermo Fisher Scientific). Twenty‐five micrograms of protein were digested using the iST kit (PreOmics). Peptides were diluted using LC–MS H2O 0.1% (v/v) formic acid to 10 ng/μL. Two hundred nanograms of peptides were loaded onto Evotips (Evosep) for purification. Pierce HeLa tryptic Digest Standard (Thermo Fisher Scientific) was also loaded for quality control.
Gonzalez‐Rodriguez M., Villar‐Conde S., Astillero‐Lopez V., Villanueva‐Anguita P., Ubeda‐Banon I., Flores‐Cuadrado A., Martinez‐Marcos A, & Saiz‐Sanchez D. (2023). Human amygdala involvement in Alzheimer's disease revealed by stereological and dia‐PASEF analysis. Brain Pathology, 33(5), e13180.
+ Open protocol
+ Expand
2

Nanoflow HPLC-MS/MS Proteomics

Check if the same lab product or an alternative is used in the 5 most similar protocols
HPLC-grade water, acetonitrile, formic acid, and Pierce HeLa tryptic digest standard were acquired from Thermo Fisher Scientific (San Jose, CA). The column was prepared in-house by polymerizing a frit from a 3:1 mixture of Kasil 1 (29.1% potassium silicate solution) from PQ Corporation (Valley Forge, PA) and formamide from Sigma-Aldrich (St. Louis, MO) inside a 75 μm internal diameter, 360 μm outer diameter polyimide-coated fused silica capillary from Polymicro/Molex (Phoenix, AZ). The frit was cut to ∼0.2 mm, and the end was polished. The fritted capillary was packed with Magic C18AQ, 3 μm diameter, 200 Å pore size beads from Michrom Bioresources (Auburn, CA) to 30 cm of stationary phase. Liquid chromatography (LC) separation was performed on an Ultimate 3500 system from Thermo Fisher Scientific, and mass spectrometry data were acquired on an LTQ Orbitrap XL ETD (Tune Plus version 2.5.5) from Thermo Fisher Scientific. The sample was electrosprayed using a distal-coated 20 μm internal diameter, 360 μm outer diameter tip with a 10 μm opening from New Objective (Woburn, MA) connected directly to the column head using a Teflon sleeve.
Kreimer S., Belov M.E., Danielson W.F., Levittsky L.I., Gorshkov M.V., Karger B.L, & Ivanov A.R. (2016). Advanced Precursor Ion Selection Algorithms for Increased Depth of Bottom-Up Proteomic Profiling. Journal of proteome research, 15(10), 3563-3573.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!