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Transscript one step gdna removal and cdna synthesis supermix at311

Manufactured by Transgene
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TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311) is a lab equipment product designed for the removal of genomic DNA (gDNA) and the synthesis of complementary DNA (cDNA) in a single reaction. This SuperMix combines gDNA removal and cDNA synthesis in a convenient one-step process.

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Lab products found in correlation

Perfectstart green qpcr supermix aq601, by Transgene (2 mentions) Triquick reagent, by Solarbio (1 mentions) Annexin 5 fitc apoptosis detection kit c1062l, by Beyotime (1 mentions) Easysep magnet, by STEMCELL (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions) Red blood cell lysis buffer, by Beyotime (1 mentions) Pitstop 2, by Abcam (1 mentions) Jetprime buffer, by Polyplus Transfection (1 mentions) Farnesyl pyrophosphate ammonium salt, by Merck Group (1 mentions) Hexane, by Merck Group (1 mentions) Nadph, by Merck Group (1 mentions) Mir x mirna first strand synthesis and sybr qrt pcr kit, by Takara Bio (1 mentions) Lightcycler 480 real time pcr instrument, by Roche (1 mentions) Metformin phr1084, by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (407 citations) CDNA Synthesis SuperMix (94 citations) One-Step gDNA Removal and cDNA Synthesis SuperMix (66 citations) TransScript One-Step gDNA Removal (9 citations) AT311 (5 citations)

4 protocols using transscript one step gdna removal and cdna synthesis supermix at311

1

Mouse CD8+ T Cell Isolation and Apoptosis

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Propidium iodide solution (PI; B316099) was purchased from BioLegend (CA, USA). Lymphocyte Separation Medium (P8620) and TriQuick Reagent (R1100) were purchased from Solarbio (Beijing, China). The EasySep™ Mouse CD8+ T Cell Isolation Kit (19753) and EasySep™ Magnet (18000) were purchased from StemCell Technologies (Vancouver, Canada). The Annexin V-FITC Apoptosis Detection Kit (C1062L) and Red Blood Cell Lysis Buffer (C3702) were purchased from Beyotime (Shanghai, China). Pitstop-2 (ab120687) was purchased from Abcam (Illinois, USA). Hydroxy-Dynasore (HY-13863) was purchased from MedChemExpress (New Jersey, USA). CellTiter-Glo® Luminescent Cell Viability Assay (G7570) was purchased from Promega (WI, USA). PerfectStartTM Green qPCR SuperMix (AQ601) and TransScript® one-step gDNA removal and cDNA synthesis Supermix (at311) were purchased from TransGen Biotech (Beijing, China). The transfection reagent jetPRIME (25Y1801N5) and jetPRIME buffer (B210217) were purchased from Polyplus Transfection (Illkirch Graffenstaden, France).
Wei M., Mo Y., Liu J., Zhai J., Li H., Xu Y., Peng Y., Tang Z., Wei T., Yang X., Huang L., Shao X., Li J., Zhou L., Zhong H., Wei C., Xie Q., Min M, & Wu F. (2022). Ubiquitin ligase RNF125 targets PD-L1 for ubiquitination and degradation. Frontiers in Oncology, 12, 835603.
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2

Farnesyl pyrophosphate ammonium salt extraction

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Farnesyl pyrophosphate ammonium salt, hexane, and NADPH were purchased from Sigma-Aldrich (St. Louis, MO, USA). The RNA isolation kit (DP441) was obtained from Tiangen (Beijing, China). Isopropyl β-d-thiogalactopyranoside (IPTG) was purchased from Coolaber (Beijing, China). The TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311) was obtained from TransGen Biotech (Shanghai, China).
Chen F.Y., Mu Q.Y., Xu B.Y., Lei Y.C., Liu H.Y, & Fang X. (2024). Functional analysis of CYP71AV1 reveals the evolutionary landscape of artemisinin biosynthesis. Frontiers in Plant Science, 15, 1361959.
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3

Optimized RNA Extraction and qRT-PCR Analysis

Cited 1 time
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Total RNA was extracted using a modified CTAB method (Porebski et al. 1997 (link)). The total RNA sample was treated with DNase I to remove any contaminating genomic DNA, and then quantified.
For miRNA qRT-PCR (Quantitative reverse transcription PCR) analyses, miRNA first-Strand was synthesized using the Mir-X™ miRNA First-Strand Synthesis and SYBR® qRT-PCR Kit according to the instructions (638,313, Takara Biotechnology Co., Ltd). For target mRNA qRT-PCR analyses, first-strand cDNA was synthesized using TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311, Transgene, Beijing, China) (Pei et al. 2020 (link)).
The qPCR experiment was performed on LightCycler 480 Real-Time PCR instrument (Roche Diagnostics®, Basel, Switzerland). All reactions were performed with three replications, and U6 snRNA and PyTUB were respectively used as the internal control for miRNA and mRNA. Relative expression level of each gene was calculated using the 2-ΔΔCp algorithm. The qPCR primers are listed in Supplementary Table S2.
Liu H., Shu Q., Lin-Wang K., Allan A.C., Espley R.V., Su J., Pei M, & Wu J. (2021). The PyPIF5-PymiR156a-PySPL9-PyMYB114/MYB10 module regulates light-induced anthocyanin biosynthesis in red pear. Molecular Horticulture, 1, 14.
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4

Metformin and Tyloxapol Effects on Metabolism

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Metformin (PHR1084) and tyloxapol (T8761) were purchased from Sigma-Aldrich (MO, USA). Glucose (20171108) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). A blood glucose meter (06656919032) and test strips (1072332990) were purchased from Roche (Basel, Switzerland). Dulbecco’s modified Eagle’s medium (DMEM, high-glucose, D5796) was purchased from Millipore (MA, USA). Lipofectamine 2000 (11668027) was purchased from Invitrogen (Carlsbad, CA, USA). PerfectStartTM Green qPCR SuperMix (AQ601) and TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix (AT311) were purchased from TransGen Biotech (Beijing, China). Biochemical kits for mouse AST (200218), ALT (191230), TG (200224) and CHO (200224) were purchased from Ruierda Biological Technology (Beijing, China). ELISA kits for mouse ApoB (SEC003Mu), ApoB100 (PAA603Mu01), albumin (CEB028Mu), ApoE (SEA704Mu), ApoA1 (SEA519Mu), TG (CEB687Ge), and CHO (CEB701Ge) were purchased from Cloud-Clone Corp. (Wuhan, China). ELISA kits for mouse TGF-β (121702), IFN-γ (120062), IL-1β (1210122), and IL-6 (1210602) were purchased from Dakewe Biotech Co., Ltd. (Beijing, China). ELISA kits for human leptin (SEA084Hu) and adiponectin (SEA605Hu) were from Cloud-Clone Corp (Wuhan, China).
Peng Y., Zeng Q., Wan L., Ma E., Li H., Yang X., Zhang Y., Huang L., Lin H., Feng J., Xu Y., Li J., Liu M., Liu J., Lin C., Sun Z., Cheng G., Zhang X., Liu J., Li D., Wei M., Mo Y., Mu X., Deng X., Zhang D., Dong S., Huang H., Fang Y., Gao Q., Yang X., Wu F., Zhong H, & Wei C. (2021). GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity. Nature Communications, 12, 7004.
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