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Rabbit anti human cd163 antibodies

Manufactured by Enzo Life Sciences

Rabbit anti-human CD163 antibodies are a laboratory reagent used for the detection and analysis of the CD163 protein in human samples. CD163 is a cell surface receptor expressed on various immune cells, including macrophages. These antibodies can be utilized in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study the expression and distribution of CD163 in different biological contexts.

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2 protocols using rabbit anti human cd163 antibodies

1

Phenotyping of Human BALF Macrophages

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Human BALF cell cytospin slides were fixed in 4% paraformaldehyde, incubated in blocking buffer (Aurion Blocking Solution, Electron Microscopy Sciences, Hatfield, PA) and reacted overnight with mouse anti-human CD68 (1:50 dilution, R & D Systems, Minneapolis, MN) and rabbit anti-human CD163 antibodies (1:50 dilution, Enzo Life Sciences, Inc., NY) diluted in 0.1% Aurion BSA-c (Electron Microscopy Sciences). Slides were washed 5 times in PBS, incubated with species-specific secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 568 (Life Technologies, NY) at a 1:200 dilution, washed 5 times, mounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA) and visualized utilizing a Zeiss LSM 510 UV confocal microscope (Carl Zeiss Microscopy GmbH, Jena, Germany).
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2

Phenotyping of Human BALF Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human BALF cell cytospin slides were fixed in 4% paraformaldehyde, incubated in blocking buffer (Aurion Blocking Solution, Electron Microscopy Sciences, Hatfield, PA) and reacted overnight with mouse anti-human CD68 (1:50 dilution, R & D Systems, Minneapolis, MN) and rabbit anti-human CD163 antibodies (1:50 dilution, Enzo Life Sciences, Inc., NY) diluted in 0.1% Aurion BSA-c (Electron Microscopy Sciences). Slides were washed 5 times in PBS, incubated with species-specific secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 568 (Life Technologies, NY) at a 1:200 dilution, washed 5 times, mounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA) and visualized utilizing a Zeiss LSM 510 UV confocal microscope (Carl Zeiss Microscopy GmbH, Jena, Germany).
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