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Ab19331

Manufactured by Abcam
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Ab19331 is a recombinant protein used as a positive control for western blotting. It is produced in a human cell line and purified using standard biochemical techniques.

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Lab products found in correlation

Sc 584, by Santa Cruz Biotechnology (1 mentions) C15400001, by Diagenode (1 mentions) C15410206, by Diagenode (1 mentions) Ab76228, by Abcam (1 mentions) Ab195436, by Abcam (1 mentions) Ab50517, by Abcam (1 mentions) Ab34710, by Abcam (1 mentions) P jak2, by Cell Signaling Technology (1 mentions) Sc 28298, by Santa Cruz Biotechnology (1 mentions) Sc 518007, by Santa Cruz Biotechnology (1 mentions) Adi spa 836 f, by Enzo Life Sciences (1 mentions) P erk 4376, by Cell Signaling Technology (1 mentions) Sc 271511, by Santa Cruz Biotechnology (1 mentions) Ab5694, by Abcam (1 mentions)

2 protocols using ab19331

1

Chromatin Immunoprecipitation Assay Protocol

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ChIP assays were performed as described previously.69 (link) The antibodies used for ChIP assay includes normal IgG mouse (C15400001) or rabbit (C15410206, Diagenode, Seraing, Belgium), ERα (8644) and acetylated histone 3 (9649, CST, Danvers, MA, USA), ERβ (2780-1) and ERRβ (ab19331, Abcam), p300 (sc-584) and Pol II (sc-899, Santa Cruz, Dallas, TX, USA). The immunoprecipitated DNA samples were PCR amplified using primers mentioned in Supplementary Table 1.
Chaudhary S., Madhukrishna B., Adhya A.K., Keshari S, & Mishra S.K. (2016). Overexpression of caspase 7 is ERα dependent to affect proliferation and cell growth in breast cancer cells by targeting p21Cip. Oncogenesis, 5(4), e219-.
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2

Antibody-based Protein Expression Analysis

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Antibodies were used against the following proteins: Sirt1 (ab50517), Sirt5 (ab195436), ERRα (ab76228), ERRβ (ab19331), α-SMA (ab5694), collagen 1A1 (ab34710, Abcam); Sirt2 (sc-28298), SHP (sc-271511), and CYP7A1 (sc-518007, Santa Cruz Biotechnology); Sirt4 (3224) and Sirt7 (3099, Biovision); Sirt3 (5490s), Sirt6 (12486s), Ac-lysine (Ac-K) (9441s), p-STAT3 (Y705) (9145s), STAT3 (12640s), Bax (2772s), cleaved caspase-3 (9664s), p-ERK (4376s), ERK (4695s), p-JNK (4671s), JNK (9258s), p-p38 (9211s), p38 (9212s), and p-JAK2 (4406s; Cell Signaling Technology); Bcl2 (BS1511) and GAPDH (AP0066, Bioworld Technology); ERRγ (PP-H6812-00, Perseus Proteomics); or HSP90 (ADI-SPA-836-F, Enzo Life Sciences).
Hao L., Bang I.H., Wang J., Mao Y., Yang J.D., Na S.Y., Seo J.K., Choi H.S., Bae E.J, & Park B.H. (2020). ERRγ suppression by Sirt6 alleviates cholestatic liver injury and fibrosis. JCI Insight, 5(17), e137566.
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