1H NMR spectra were collected on a Varian Unity INOVA-300 spectrometer with TMS as the internal standard. 13C NMR spectra were recorded on a Varian Unity INOVA-300 spectrometer at 75 MHz. MALDI-TOF measurements were recorded on a Bruker Autoflex Speed spectrometer with DCTB as the matrix. UV–Vis spectra were recorded on a UV-1800 Shimadzu spectrometer. CD measurements were performed on a Jasco J-1500 circular dichroism spectrometer, equipped with a PFD-425S/15 Peltier-type temperature controller. Solution excitation and steady-state fluorescence emission spectra were recorded on a FluoroMax-4 spectrofluorometer (Horiba Scientific) and analyzed with an Origin (v8.0) integrated software FluoroEssence (v2.2). TEM images were performed on a Tecnai G2 Spirit BioTWIN electron microscope (acceleration voltage: 120 kV). Electron paramagnetic resonance (EPR) measurements were performed on a JEOL JES-FA200 apparatus, with the utilization of 2,2,6,6-tetra-methylpiperidine as the spin trap for 1O2. Fluorescence microscopy images were performed on an inverted fluorescence microscope (Olympus IX81).
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