The secondary metabolites used in this study (1 –20 , Figure 1 ) have been isolated from pathogenic fungifollowing procedures previously reported and listed in Table 1 . All the data regarding their source, their chemical family and literature are reported in Table 1 . The purity of each compound was >98%, as ascertained by TLC, ESI-MS and NMR using well-established methods. Analytical and preparative thin-layer chromatography (TLC) was performed on silica gel (Kieselgel 60, F254, 0.25 and 0.5 mm respectively) plates (Merck, Darmstadt, Germany); the spots were visualized by exposure to UV light or by spraying with 10% H2SO4 in CH3OH and then 5% phosphomolybdic acid in EtOH, followed by heating at 110 °C for 10 min. ESI-MS spectra were recorded on Agilent Technologies 6120 quadrupole LC/MS instrument (Agilent instruments, Milan, Italy); 1H NMR spectra were recorded at 400 MHz, on Bruker spectrometer (Bruker BioSpin GmbH., Karlsruhe, Germany), using the same solvent as an internal standard.
6120 quadrupole lc ms instrument
Manufactured by Agilent Technologies
Sourced in Italy
The 6120 Quadrupole LC/MS instrument is a liquid chromatography-mass spectrometry (LC/MS) system designed for the analysis of a wide range of compounds. It provides high-performance mass spectrometry capabilities coupled with a robust and reliable liquid chromatography system.
Automatically generated - may contain errors
3 protocols using 6120 quadrupole lc ms instrument
1
Isolation and Characterization of Fungal Metabolites
2
Purification and Characterization of Organic Compounds
3
Characterization of Chemical Compounds
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Optical rotations were measured in MeOH on a P-1010 digital polarimeter (Jasco, Tokyo, Japan), unless otherwise noted. IR spectra were recorded as a glass film deposits using a 5700 FT-IR spectrometer (Jasco), and UV spectra were measured in MeCN on a V-530 spectrophotometer (Easton). 1 H and 13 C NMR spectra were recorded, respectively, at 400 and 100 MHz in CDCl 3 , on a Bruker spectrometer (Billerica), using the same solvent as internal standard. The multiplicities were determined by DEPT spectrum (Berger and Braun, 2004) . COSY, HSQC, HMBC and NOESY spectra were recorded using Bruker microprograms. HR ESIMS spectra were recorded on a 6120 Quadrupole LC/MS instrument (Agilent Technologies). Analytical (0.25 mm thickness) and preparative TLC (0.50 mm thickness) were performed on silica gel (Kieselgel 60, F 254 ,) and on reversed phase (Kieselgel 60 RP-18, F 254 , 0.20 mm tickness) plates (Merck). Resulting spots were visualized by exposure to UV radiation (253 nm), or by spraying first with 10% H 2 SO 4 in MeOH and then with 5% phosphomolybdic acid in EtOH, followed by heating at 110°C for 10 min. Column chromatography was performed using silica gel (Merck, Kieselgel 60, 0.063-0.200 mm).
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