Protein extraction and western blot analysis were performed as described previously [16 (link)]. Antibodies against CAV1 (Cell Signaling Technology, Danvers, MA, USA)), cleaved caspase-3 (Cell Signaling Technology), CD63 (Abcam, Cambridge, UK)), CD81 (Cell Signaling Technology), EGFR and phosphorylated EGFR (Cell Signaling Technology), PI3K and phosphorylated PI3K (Cell Signaling Technology), Akt and pAkt Ser473 (Cell Signaling Technology), mTOR and phosphorylated mTOR (Cell Signaling Technology), and β-actin (1:10000, AC-74; Sigma-Aldrich) were used.
Mtor and phosphorylated mtor
Manufactured by Cell Signaling Technology
Sourced in United Kingdom, United States
mTOR (mammalian target of rapamycin) is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. Phosphorylated mTOR represents the active form of the enzyme. These products can be used to study the regulation and activity of the mTOR signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Pi3k and phosphorylated pi3k, by Cell Signaling Technology (2 mentions)
β actin, by Merck Group (2 mentions)
Akt and p akt ser473, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti ctsb, by Cell Signaling Technology (1 mentions)
Akt and phosphorylated akt, by Cell Signaling Technology (1 mentions)
β actin, by Techcomp Instruments (1 mentions)
Mmp 9, by Abcam (1 mentions)
2 protocols using mtor and phosphorylated mtor
1
Protein Analysis of Apoptosis Markers
2
Protein Expression Analysis Protocol
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Equal amounts of protein lysates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. Filters were probed with the following specific primary antibodies: anti-CTSB (Cell signaling, Danvers, MA, USA), MMP-9 (Abcam, Cambridge, MA, USA), β-actin (Sigma, St Louis, MO, USA), PI3K and phosphorylated-PI3K (Cell signaling, Danvers, MA, USA), Akt and phosphorylated-Akt (Cell signaling, Danvers, MA, USA), mTOR, and phosphorylated-mTOR (Cell signaling, Danvers, MA, USA). Blots were then incubated with horseradish peroxidase-conjugated secondary antibody (Pierce, Rockford, IL, USA) and visualized by chemiluminescence. The band density was quantified by densitometry using Scion Image software, and normalized to β-actin levels.
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