Tlc silica gel 60 f254 tlc plates

For the identification of EAM37 precipitates, the TLC method was developed using different mobile phases and detection reagents. TLC silica gel 60 F₂₅₄ TLC plates (20 × 20 cm, Merck, Germany) were cut into pieces (6.6 cm×3.8cm). Each TLC piece plate was marked at a distance of 1 cm from the bottom with a graphite pencil. An equal amount of each sample/ standard (0.5 μl) was loaded with a micropipette at an equal distance on the marked line and allowed to dry. TLC was performed with the laboratory available alkaloids standards, including morphine, quinine, scopolamine, caffeine, and berberine. Then these TLCs were developed in different combinations of mobile phase (15 ml) in a TLC tank. After development, TLC plates were allowed to dry and were visualized for active compounds under 254 nm and 356 nm wavelengths. Later, the TLC plates were stained with Dragendorff reagent to detect alkaloids.

For the identification of EAM37 precipitates, the TLC method was developed using different mobile phases and detection reagents. TLC silica gel 60 F₂₅₄ TLC plates (20 × 20 cm, Merck, Germany) were cut into pieces (6.6 cm×3.8cm). Each TLC piece plate was marked at a distance of 1 cm from the bottom with a graphite pencil. An equal amount of each sample/ standard (0.5 μl) was loaded with a micropipette at an equal distance on the marked line and allowed to dry. TLC was performed with the laboratory available alkaloids standards, including morphine, quinine, scopolamine, caffeine, and berberine. Then these TLCs were developed in different combinations of mobile phase (15 ml) in a TLC tank. After development, TLC plates were allowed to dry and were visualized for active compounds under 254 nm and 356 nm wavelengths. Later, the TLC plates were stained with Dragendorff reagent to detect alkaloids.