Genomic DNA was extracted from peripheral blood from 16 patients (all except patient #13) and 5 healthy controls using QIAamp blood kits (Qiagen, Valencia, CA, USA). TaqMan® predesigned primers and probes (Applied Biosystems, Foster city, CA, USA) were used for rs28940579 (V726A), rs28940578 (M694I), rs28940580 (M680I), and rs37433930 (E148Q) in MEFV, rs2066844 (R702W) and rs2066845 (G908R) in NOD2/CARD15, rs4149584 (R92Q), rs34751757 (T90N), and rs1419637 (P46L) in TNFRSF1A. Restriction fragment length polymorphism (RFLP) method was used to genotype rs61752717 (M694V) in MEFV (20 (link)) and rs2066847 (3020InsC) in NOD2/CARD15 as previously described (21 (link)).
Taqman predesigned primers and probes
Manufactured by Thermo Fisher Scientific
Sourced in United States
TaqMan® predesigned primers and probes are a collection of ready-to-use primers and probes designed for real-time PCR applications. They provide a standardized and optimized solution for gene expression analysis, genotyping, and other nucleic acid detection and quantification assays.
Automatically generated - may contain errors
Lab products found in correlation
Qiaamp blood kit, by Qiagen (1 mentions)
Abi stepone plus sequence detection system, by Thermo Fisher Scientific (1 mentions)
Taqman gene expression master mix and assays, by Thermo Fisher Scientific (1 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
2 protocols using taqman predesigned primers and probes
1
Genetic Profiling of Familial Inflammatory Disorders
2
Quantitative RT-PCR for Gene Expression Analysis
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Total RNA was extracted using RNeasy Mini Kit (Qiagen), and RNA was reverse transcribed into cDNA using High Capacity cDNA Reverse Transcription Kits (Applied Biosystems). cDNA was diluted 5 fold with nuclease-free water and quantitated via TaqMan Gene Expression Assays and Master Mix (Applied Biosystems, Carlsbad, California). TaqMan pre-designed primers and probes (Applied Biosystems) were used for target gene qPCR. PCR amplifications were performed using the ABI Step One Plus Sequence Detection System (Applied Biosystems) under standard conditions. Transcript levels were determined by standard curve and normalized to corresponding β2m levels.
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