Anti tlr7

Manufactured by Abcam

Anti-TLR7 is a laboratory reagent used in research applications. It is an antibody that specifically binds to the Toll-like receptor 7 (TLR7) protein. TLR7 is a pattern recognition receptor involved in the immune response. The core function of Anti-TLR7 is to enable the detection and study of TLR7 expression and activity in experimental settings.

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Rabbit anti-TLR7 (3 citations) Anti-TLR7 antibody (1 citations)

3 protocols using anti tlr7

Regulation of IRF7 Signaling Pathway

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CpGA (ODN2116), CpGB (ODN2006) and R848 were purchased from Invivogen. The following antibodies were used for immunoblotting: anti-MYC, anti-IRF7, and anti-IRAK1 (Santa Cruz Biotech); anti-TLR7 (Abcam); anti-MyD88 and anti-TLR9 (eBioscience); anti-STAT1, anti-NCOR2, anti-HDAC1, anti-HDAC2, anti-HDAC3, anti-HDAC4, anti-HDAC6, anti-HDAC7, anti-pIKKα/β, anti-pIκBα, anti-IκBα, anti-pJNK, anti-pp38, anti-TBK1, anti-pTBK1 and anti-pERK (Cell Signaling Technologies); and anti-GAPDH (Sigma).
NE-PER Nuclear and Cytoplasmic Extraction Reagents (Pierce) was used to investigate nuclear translocation of IRF7 as per the manufacturer’s instructions. MYC inhibitor (c-Myc Inhibitor II - CAS 413611-93-5) was purchased from CalBiochem. HDAC inhibitors, valproic acid (VPA) was purchased from Invivogen.

Kim T.W., Hong S., Lin Y., Murat E., Joo H., Kim T., Pascual V, & Liu Y.J. (2016). Transcriptional repression of IRF7 by MYC is critical for type I interferon production in human pDC. Journal of immunology (Baltimore, Md. : 1950), 197(8), 3348-3359.

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Immunological Reagents for Signaling Pathways

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Anti-TLR7 (against N-terminal for WB), anti-ATF4 and anti-Xbp1 were from Abcam. Anti-TLR7 (against C-terminal for WB) was purchased from Cell Signaling. Anti-TLR7 (against N-terminal) from Invitrogen, Anti-TLR7 (against C-terminal) and Anti-TLR7 (against C-terminal for IF) from Noves Biologicals. Anti-Pin1 and anti-ATF6 from Santa Cruz Biotechnology. SYBR Green PCR Master Mix was from Bio-Rad. R848, OVA and anti-β-actin were from Sigma. PCR primers were purchased from IDT, Inc. Protease Inhibitor Mixture was from Calbiochem. The DyLight 800/680 secondary antibodies and IMJECT Alum were purchased from Thermo Scientific. TaqMan Universial PCR Master Mix (for Rhinovirus) and TaqMan Gene Expression Assay (for SeV) were from Applied Biosystem. Sendai virus was from ATCC. Mouse TLR1–9 Agonist kit was from InvivoGen. Murine IL-5, mSCF and mFLT3L were from PeproTech. EDN (RNase2) ELISA kit, MBP (Prg2) ELISA kit and ECP ELISA kit were from MyBioSource. Anti-PRG2, anti-ECP and anti-EDN were from Biorbyt. LEGENDplx Mouse Proinflammatory Chemokine Panel was from Biolegend.

Shen Z.J., Hu J., Kashi V., Bochkov Y.A., Gern J.E, & Malter J.S. (2018). TLR-7 stress signaling in differentiating and mature eosinophils is mediated by the prolyl isomerase Pin1. Journal of immunology (Baltimore, Md. : 1950), 201(12), 3503-3513.

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Western Blot Protein Expression Analysis

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Western Blot was performed to measure protein expressions as previously described. 1 (link) Protein samples (50 μg) were loaded on a Tris-glycine gel, electrophoresed, and transferred to a nitrocellulose membrane. Membranes were incubated overnight at 4°C with the primary antibodies: rabbit polyclonal anti-TLR7 (1:5000; Abcam, Cambridge, MA), rabbit monoclonal anti-BTK (1:4000; Cell Signaling, Danvers, MA), rabbit polyclonal anti-CRT (1:5000; Abcam), rabbit polyclonal anti-LRP1 (1:300000, Abcam), and rabbit polyclonal anti-Hx (1:2000; Abcam). The same membrane was probed with an antibody against β-actin (1:5000; Santa Cruz Biotechnology, Santa Cruz, CA) or tubulin (1:5000; Santa Cruz Biotechnology) as an internal control after being stripped. Incubation with secondary antibodies (Santa Cruz Biotechnology) was done for 1 hour at room temperature. Immunoblots were then probed with an enhanced chemiluminescence (ECL Plus reagent kit; Amersham Biosciences, Arlington Heights, IL) and visualized with an imaging system (Versa Doc, Model 4000; Bio-Rad Laboratories, Hercules, CA). Data were analyzed using Image J software.

Wang G., Guo Z., Tong L., Xue F., Krafft P.R., Budbazar E., Zhang J.H, & Tang J. (2018). TLR7 (Toll-Like Receptor 7) Facilitates Heme Scavenging Through the BTK (Bruton Tyrosine Kinase)-CRT (Calreticulin)-LRP1 (Low-Density Lipoprotein Receptor-Related Protein-1)-Hx (Hemopexin) Pathway in Murine Intracerebral Hemorrhage. Stroke, 49(12).

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