Vector dab peroxidase substrate

Manufactured by Vector Laboratories

Vector DAB Peroxidase substrate is a chromogenic substrate used in immunohistochemistry to detect the presence of target proteins in tissue samples. It produces a brown, insoluble reaction product when catalyzed by peroxidase-conjugated antibodies. This substrate is commonly used in various biological and medical research applications.

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Lab products found in correlation

Vectastain elite abc, by Vector Laboratories (1 mentions) Shandon coverplates, by Thermo Fisher Scientific (1 mentions) Permount, by Thermo Fisher Scientific (1 mentions) Ab15200, by Abcam (1 mentions) Goat serum, by Thermo Fisher Scientific (1 mentions) Vectastain universal elite abc kit, by Vector Laboratories (1 mentions) Ab18256, by Abcam (1 mentions)

Close spelling variants of this product

Vector DAB Peroxidase Substrate Kit Vector DAB peroxidase (HRP) substrate kit Vector DAB substrate kit for peroxidase Vector DAB substrate DAB peroxidase substrate DAB substrate Vector® DAB

2 protocols using vector dab peroxidase substrate

CD8+ T Cell Immunohistochemistry

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Frozen tissue was embedded in Frozen Section Medium (Azer Scientific), cut on a cryostat (5 um), and mounted onto Superfrost Plus glass microscope slides. Tissue was fixed on slides with acetone (−20 °C) and endogenous peroxidase activity was blocked with 0.6 % hydrogen peroxide. Slides were placed on Shandon coverplates™ (Thermo Fisher) using 0.2 % Triton-× 100/PBS and placed in a Sequenza™ slide rack (Thermo Fisher). Slides were incubated with Biotin conjugated rat anti-mouse CD8 (eBioscience, 1:50 in 1%BSA/PBS), or diluent as negative control, and then with Vectastain Elite ABC (Vector Laboratories, Burlingame, CA), developed with Vector DAB Peroxidase substrate (Vector Laboratories) and mounted with Permount (Thermo Fisher).

Zervos E., Agle S., Freistaedter A.G., Jones G.J, & Roper R.L. (2016). Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer. Journal of Experimental & Clinical Cancer Research : CR, 35, 39.

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Immunohistochemical Analysis of Skeletal Muscles

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OCT-embedded skeletal muscles, kidney and liver sections on glass coverslips were washed in PBS, fixed in 4% paraformaldehyde, quenched with 50 mM glycine, permeabilized with 0.3% Triton X-100 in PBS and saturated with 3% goat serum (Thermo, 50062Z). Vectastain Elite ABC universal kit-haematoxylin counterstain (Vector Laboratories, PK-6200) with Vector DAB Peroxidase Substrate (Vector Laboratories, SK-4100) was used following the manufacturer’s instructions. Primary antibodies were used for phosphor-histone H2A.X (1:400; Cell Signaling, 9718), HMGB1 (1:1,000; Abcam, ab18256) and Desmin (1:200; Abcam, ab15200).

Jeon O.H., Mehdipour M., Gil T.H., Kang M., Aguirre N.W., Robinson Z.R., Kato C., Etienne J., Lee H.G., Alimirah F., Walavalkar V., Desprez P.Y., Conboy M.J., Campisi J, & Conboy I.M. (2022). Systemic induction of senescence in young mice after single heterochronic blood exchange. Nature metabolism, 4(8), 995-1006.

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