Recombinant mil 2

Manufactured by R&D Systems

Recombinant mouse Interleukin-2 (mIL-2) is a cytokine produced by T cells that promotes the growth and differentiation of T cells, B cells, and natural killer cells. It is a recombinant protein expressed in E. coli.

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Lab products found in correlation

Mil 7, by R&D Systems (2 mentions) Plzf 9e12, by BioLegend (1 mentions) Fluorochrome conjugated streptavidin, by BD (1 mentions) Gata3 l50 823, by BD (1 mentions) Mil 33, by R&D Systems (1 mentions) Mil 25, by R&D Systems (1 mentions) Ra3 6b2, by BD (1 mentions) Sulprostone, by Cayman Chemical (1 mentions) Ono ae3 208, by Cayman Chemical (1 mentions) Pge1 alcohol, by Cayman Chemical (1 mentions) Butaprost, by Cayman Chemical (1 mentions) Ah6809, by Cayman Chemical (1 mentions) Dibutyryl camp db camp, by Merck Group (1 mentions) Streptavidin microbeads, by Miltenyi Biotec (1 mentions) Lympholyte m, by Cedarlane (1 mentions)

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MIL-2 (5 citations)

3 protocols using recombinant mil 2

Multiparametric Flow Cytometry Analysis of Mouse Immune Cell Subsets

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For flow-cytometric analyses, mAbs specific for mouse CD3ε (145-2C11), CD8α (53–6.7), CD19 (1D3), B220 (RA3-6B2), NK1.1 (PK136), CD11b (M1/70), CD11c (HL3), Gr-1 (RB6-8C5), TER119 (TER119), CD45.1 (A20), CD45.2 (2F1), Sca-1 (E13-161.7), CD25 (PC61), Thy1.2 (53–2.1), Flt3 (A2F10), α4β7 (DATK32), KLRG1 (2F1), CCR9 (CW-1.2), CD31 (MEC13.3), GATA3 (L50-823), T-bet (O4-46), RORγt (Q31-378), IFN-γ (XMG1.2), IL-17A (TC11-18H10), and fluorochrome-conjugated streptavidin were purchased from BD. mAbs against mouse Notch1 (HMN1-12), Notch2 (HMN2-35), CD4 (GK1.5), PDGFRα (ATA5), gp38 (8.1.1), and PLZF (9E12) were purchased from BioLegend. mAbs against c-Kit (2B8), FcεRIα (MAR-1), IL-7Rα (A7R34), and IL-13 (eBio13A) were purchased from eBioscience. Anti-T1/ST2 (DJ8) was purchased from MD Biosciences. mAb against mouse CD16/CD32 (2.4G2) was purified from hybridoma culture supernatants in our laboratory.
Recombinant mIL-2, mIL-7, mIL-25, mIL-33, and mTSLP were purchased from R&D Systems. A STAT5 inhibitor (CAS 285986–31-4) was purchased from Calbiochem, and Dox was purchased from Clontech.

Koga S., Hozumi K., Hirano K.I., Yazawa M., Terooatea T., Minoda A., Nagasawa T., Koyasu S, & Moro K. (2018). Peripheral PDGFRα+gp38+ mesenchymal cells support the differentiation of fetal liver–derived ILC2. The Journal of Experimental Medicine, 215(6), 1609-1626.

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Multiparametric Flow Cytometry Profiling

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Biotin-conjugated anti-CD4 (GK1.5), CD8 (53-6.7), CD5 (53-7.3), TCRβ (H57-597), CD11b (M1/70), CD11c (N418), Gr-1 (RB6-8C5), B220 (RA3-6B2), TER119 (TER-119), FcεRIα (MAR-1), APC-conjugated anti-ST2 (RMST2-2), PE-conjugated anti-CD25 (PC61.5), PerCP-Cyanine5.5-conjugated anti-IL-5 (TRFK5), PE-Cyanine7-conjugated GATA-3 (TWAJ), and PE-Cyanine5.5-conjugated anti-IL-13 (eBio13A) were purchased from eBioscience. PE-conjugated anti-CD25 (7D4) and PerCP-Cyanine5.5-conjugated anti-CD45 (30-F11) were purchased from BD Pharmingen. FITC-conjugated and APC-conjugated streptavidin, recombinant mIL-33 were purchased from BioLegend. Recombinant mIL-2 and mIL-7 were purchased from R&D. APC-conjugated anti-ST2 (DJ8) was purchased from MD Bioproducts. PE-conjugated anti-Siglec-F (ES22-10D8) and Streptavidin MicroBeads were purchased from MiltenyiBiotec. PGE₂, sulprostone, butaprost, PGE₁-alcohol, AH6809, and ONO-AE3-208 were purchased from Cayman Chemical. Dibutyryl cAMP (db-cAMP) was purchased from Sigma-Aldrich.

Zhou Y., Wang W., Zhao C., Wang Y., Wu H., Sun X., Guan Y, & Zhang Y. (2018). Prostaglandin E2 Inhibits Group 2 Innate Lymphoid Cell Activation and Allergic Airway Inflammation Through E-Prostanoid 4-Cyclic Adenosine Monophosphate Signaling. Frontiers in Immunology, 9, 501.

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In Vitro Priming and Adoptive Transfer of DO11.10 T Cells

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Wild-type DO11.10 T cells were primed in vitro as previously described (Dooms et al., 2004 (link)). Briefly, CD4⁺ T cells were isolated from lymph nodes and spleens of WT DO11.10 mice using CD4⁺ Dynal beads (Dynal, Oslo, Norway) and 2.5 × 10⁵ CD4⁺ T cells were primed with 2.5 × 10⁶ mitomycin C-treated BALB/c splenocytes and 1 μg/ml OVA_323–339 for 4 days. T cells were harvested on day 4 and, after removal of dead cells by density gradient centrifugation (Lympholyte-M, Cedarlane Laboratories), equal numbers of KJ1–26⁺CD4⁺ T cells were adoptively transferred into unmanipulated BALB/c mice by tail vein injection. For experiments with retroviral transduction of WT and DN STAT5 at the later stage of the T cell response, DO11.10 cells were harvested on day 4 after in vitro priming and further cultured for an additional 3–5 days in the presence of 10 ng/ml recombinant mIL-2 (R&D Systems).

Fleury M., Vazquez-Mateo C., Hernandez-Escalante J, & Dooms H. (2021). PARTIAL STAT5 SIGNALING IS SUFFICIENT FOR CD4+ T CELL PRIMING BUT NOT MEMORY FORMATION. Cytokine, 150, 155770.

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