Cells were seeded into 12-wells plates at a density of 1 × 104/wells and incubated with MN (12.5 μM) and HK (12.5 μM), individually, at 37 °C for 24 h and 48 h [42 (link)]. After the supernatant was removed, methanol was added and stood for 10 min. After washing, 0.1% Triton X-100 was incubated for 10 min, washed 3 times with PBS, and incubated with 1% bovine serum albumin (BSA) for 1 h. Cells were then washed 3 times in PBS, and then incubated with primary antibody overnight at 4 °C. After washing, cells were incubated with the DyLight™ 488-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA) at room temperature for 2 h. After washing, cells were incubated with the 4′,6-diamidino-2-phenylindole (DAPI) for 10 min in the dark. The imaging of cells was acquired by using confocal laser scanning microscopy (Nikon, TE2000-U, Tokyo, Japan).
Dylight 488 conjugated goat anti rabbit igg antibody
Manufactured by Jackson ImmunoResearch
Sourced in United States
DyLight™ 488-conjugated goat anti-rabbit IgG antibody is a secondary antibody that binds to rabbit immunoglobulin G (IgG) and is conjugated with the DyLight™ 488 fluorescent dye. This antibody can be used for various immunodetection techniques, such as Western blotting, immunohistochemistry, and flow cytometry.
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2 protocols using dylight 488 conjugated goat anti rabbit igg antibody
1
Fluorescence Imaging of Cell Response
2
Immunofluorescence Staining of RAW264.7 Cells
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The treatment of RAW264.7 cells as described above. The supernatant in the plate was removed, following added methanol and wait 10 min then washed with PBS third. The 0.1% Triton X-100 was added and reacted for 10 min, following washed the cells with PBS third, and reacted with 1% bovine serum albumin (BSA) for 1 h. The cells were washed 3 times in PBS and then incubated at 4 °C overnight with primary antibody. The cells were washed 3 times with PBS and incubated with the DyLight™ 488-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch, West Grove, PA) at room temperature for 2 h. After washing the cells with PBS third, the cells were incubated in the presence of 4′,6-diamidino-2-phenylindole (DAPI) for 10 min in the dark. The imaging of cells was acquired by a confocal laser scanning microscope (Leica, TCS SP2, Germany).
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