Gpc 50

Size exclusion chromatography was carried out using a Polymer Laboratories GPC 50 instrument fitted with a differential refractive index detector. The number-average molar mass (M_n), weight average molar mass (M_w) and polydispersity (Đ, M_w/M_n) were measured by SEC using HPLC-grade chloroform as eluent at 30 °C with 1 mL min⁻¹ flow rate. PLgel guard column (50 × 7.5 mm, 8 μm) followed by a pair of PLgel Mixed-D columns (300 × 7.5 mm, 8 μm) were used for separation of the sample. Polystyrene standards of known M_n and Đ in the range of 100 Da– 500 kDa were used to calibrate the column. Molar mass and polydispersity were calculated using Polymer Labs Cirrus 3.0 software [18 ].

The analysis was
performed using triple detection conditions with either a GPC50 (Polymer
Laboratories, UK; THF mobile phase) or a Malvern OmniSec System (Malvern,
UK; DMF mobile phase). Molecular weight and molecular weight distribution
of PPS polymers were determined using a PLgel 5 μm MIXED-D column
in series with a PLgel 3 μm MIXED-E operating online at 30 °C.
THF was used as an eluent at a flow rate of 1.0 mL/min. Two linear
polystyrene standards (Malvern, UK) for calibration (narrow) and for
verification (broad) were used for the analysis of the polymers. Block
copolymers of PPS with polyacrylamides were analyzed using a D3000
column operating online at 50 °C with HPLC grade DMF containing
0.1% LiBr as the mobile phase at a flow rate of 0.8 mL/min. Calibration
was performed using a poly(methyl methacrylate) standard.