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Anti cd31 rat monoclonal antibody clone mec13.3

Manufactured by BD

Anti-CD31 rat monoclonal antibody (clone MEC13.3) is a laboratory reagent used to detect the presence of the CD31 protein, also known as PECAM-1, which is expressed on the surface of endothelial cells. This antibody can be used in various immunological techniques to identify and study cells that express CD31.

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2 protocols using anti cd31 rat monoclonal antibody clone mec13.3

1

Immunoblotting and Immunofluorescence Antibody Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monoclonal anti-Flag antibody M2 (catalogue number F1804) (dilution 1:1000) is from Sigma, anti-tubulin antibody was prepared from an anti-tubulin hybridoma clone (12G10) purchased from the Hybridoma Bank at the University of Iowa (1:10000). Anti-HA antibody was purified from 12CA5 hybridoma28 (link) (1:20000). p-JNK (#4668) (1:1000), p-ERK1/2 (#9106) (1:2000), anti-VEGFR2 (#2472) (1:1000), p-p38 (#9211) (1:1000), MLC2pT18/S19 (#3674) (1:1000 for immunoblot, 1:200 for IF), MLC2pS19 (#3671) (1:1000) anti-mouse IgG and HRP-linked Antibody (#7076) (1:5000) were purchased from Cell Signaling Technology (MA). Anti-MEKK3 is from R&D (MN) (MAB6095) (1:1000), and anti-VE-Cadherin is from Santa Cruz Biotechnology (Dallas, TX) (sc-79818) (1:200), anti-α-smooth muscle actin mouse monoclonal antibody (clone 1A4) is from Sigma (A5228) (1:200) and anti-CD31 rat monoclonal antibody (clone MEC13.3) is from BD bioscience (553370) (1:200).
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2

Immunoblotting and Immunofluorescence Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monoclonal anti-Flag antibody M2 (catalogue number F1804) (dilution 1:1,000) is from Sigma, anti-tubulin antibody was prepared from an anti-tubulin hybridoma clone (12G10) purchased from the Hybridoma Bank at the University of Iowa (1:10,000). Anti-HA antibody was purified from 12CA5 hybridoma27 (link) (1:20,000). p-JNK (#4668) (1:1,000), p-ERK1/2 (#9106) (1:2,000), anti-VEGFR2 (#2472) (1:1,000), p-p38 (#9211) (1:1,000), MLC2pT18/S19 (#3674) (1:1,000 for immunoblot, 1:200 for IF), MLC2pS19 (#3671) (1:1,000) anti-mouse IgG and HRP-linked Antibody (#7076) (1:5,000) were purchased from Cell Signaling Technology (MA). Anti-MEKK3 is from R&D (MN) (MAB6095) (1:1,000), and anti-VE-Cadherin is from Santa Cruz Biotechnology (Dallas, TX) (sc-79818) (1:200), anti-α-smooth muscle actin mouse monoclonal antibody (clone 1A4) is from Sigma (A5228) (1:200) and anti-CD31 rat monoclonal antibody (clone MEC13.3) is from BD bioscience (553370) (1:200).
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