Antibodies against cleaved caspase-3, cleaved caspase-8, cleaved caspase-9, AKT, phospho-Akt (p-Akt), ERK, phospho-ERK (p-ERK), JNK, phosphor-JNK (p-JNK) (Cell Signaling Technology, Boston, MA, USA); anti-p62/SQSTM1(MBL); antibody against LC3B, Curcumin, 3-methyladeine (3-MA), chloroquine diphosphate (CQ), Rapacymin, the fluorescent dye Acridine Orange (AO) and Crystal violet (Sigma-Aldrich, St. Louis, MO, USA); Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA); pre-stained protein standards (Fermentas, Lithuania); Human Annexin V Apoptosis Detection Kit and cell cycle test kit (BD Biosciences, San Jose, CA); β-actin antibody (Santa Cruz Biotechnology, USA); Senescence β-Galactosidase Staining Kit (Beyotime Biotechnology). Cur was dissolved with dimethyl sulfoxide (DMSO) and the storage concentration was 20 mM/ml; Rapacymin was solved in DMSO; 3-MA, CQ and AO were solved in ultrapure water.
Phosphor jnk p jnk
Manufactured by Cell Signaling Technology
Sourced in United States
Phosphor-JNK (p-JNK) is an antibody that specifically recognizes the phosphorylated form of c-Jun N-terminal kinase (JNK). JNK is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in cellular stress response and apoptosis. The Phosphor-JNK antibody can be used to detect the activation state of JNK in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Crystal violet, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Phospho erk p erk, by Cell Signaling Technology (1 mentions)
Pre stained protein standard, by Thermo Fisher Scientific (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Senescence β galactosidase staining kit, by Beyotime (1 mentions)
Phosphor erk p erk, by Cell Signaling Technology (1 mentions)
P38 t p38, by Cell Signaling Technology (1 mentions)
Radioimmunoprecipitation assay lysis buffer, by Beyotime (1 mentions)
2 protocols using phosphor jnk p jnk
1
Apoptosis and Autophagy Regulation Assay
2
Protein Extraction and Western Blot Analysis of Macrophage Signaling
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Total protein of macrophages was extracted using radio-immunoprecipitation assay lysis buffer according to the manufacturer's instruction (Beyotime, Shanghai, China). Proteins were separated by 10% SDS-PAGE, transferred onto polyvinylidene difluoride membranes, and incubated overnight with primary antibodies at 4°C. Rabbit polyclonal antibodies against phosphor-P38 (p-P38) (#4511T), P38 (t-P38) (#8690T), phosphor-JNK (p-JNK) (#9255S), phosphor-ERK (p-ERK) (#4370T), ERK (t-ERK) (#4695T), and glyceraldehyde-phosphate dehydrogenase (#5174T) were purchased from Cell Signaling Technology. Mouse polyclonal antibody against JNK (t-JNK) (#9252T) was also purchased from Cell Signaling Technology. Secondary antibodies were added and co-incubated for 2 h at room temperature. Protein bands were visualized using ECL detection reagent. The protein expression was quantified by densitometry and normalized to glyceraldehyde-phosphate dehydrogenase.
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