Anti cd45ra antibody

Manufactured by BD

The Anti-CD45RA antibody is a laboratory reagent used to detect and identify cells expressing the CD45RA surface antigen. It is a tool for cell analysis and identification in research applications.

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Lab products found in correlation

Anti pd 1 antibody, by BD (1 mentions) Anti cd8 antibody, by BD (1 mentions) Anti cd3 antibody, by BD (1 mentions) Anti cd45 antibody, by BD (1 mentions) Anti cd4 antibody, by BD (1 mentions) Fixable viability stain 620, by BD (1 mentions) Paint a gate pro software facscalibur, by BD (1 mentions) Anti cd90 antibody, by BD (1 mentions)

Spelling variants of this product

Anti-CD45RA (26 citations) PE mouse anti-rat CD45RA antibody (2 citations)

2 protocols using anti cd45ra antibody

Multi-Marker Flow Cytometry Analysis of Tumor-Infiltrating Lymphocytes

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Single-cell suspension obtained and stained by following mAbs: Live/dead dye (Fixable Viability stain 620, BD Biosciences, 564,996), anti-CD45 antibody (BD Biosciences, 560,178), anti-CD3 antibody (BD Biosciences, 563,800), anti-CD4 antibody (BD Biosciences, 566,392), anti-CD8 antibody (BD Biosciences, 563,821), anti-CD25 antibody (BD Biosciences, 564,467), anti-CD127 antibody (BD Biosciences, 558,598), anti-CD45RA antibody (BD Biosciences, 563,031), anti-CCR7 antibody (BD Biosciences, 562,555), anti-PD-1 antibody (BD Biosciences, 563,245), anti-LAG3 antibody (BD Biosciences, 565,774), anti-CD39 antibody (BD Biosciences, 561,444). Single cell suspensions were stained with 1 μg/sample fluorochrome-labeled antibodies for specific surface marker at 4°C for 30 min in 100 μL PBS. Stained single cell suspension of tumor tissue were processed to flow cytometry using Cytoflex LX. The data were analysis by using FlowJo V10.6.2 software.

Yang B., Li X., Zhang W., Fan J., Zhou Y., Li W., Yin J., Yang X., Guo E., Li X., Fu Y., Liu S., Hu D., Qin X., Dou Y., Xiao R., Lu F., Wang Z., Qin T., Wang W., Zhang Q., Li S., Ma D., Mills G.B., Chen G, & Sun C. (2022). Spatial heterogeneity of infiltrating T cells in high-grade serous ovarian cancer revealed by multi-omics analysis. Cell Reports Medicine, 3(12), 100856.

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Phenotypic Characterization of P3 ASCs

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P3 ASCs were resuspended following digestion with 0.25% trypsin and washed three times with phosphate-buffered saline (PBS). A minimum of 1 × 10⁵ cells were collected from each sample. Samples were incubated with Anti-CD34 antibody (NBP2-29455; Novus), Anti-CD45RA antibody (561886; BD Pharmingen), Anti-CD73 antibody (orb629433; BIORBYT), Anti-CD90 antibody (561973; BD Pharmingen), and Anti-CD105 antibody (NB500-452; Novus) in the dark at room temperature for 30 min. The samples were subjected to flow cytometry, and data were analyzed using Paint-A-Gate Pro™ software (FACSCalibur™; BD Biosciences).

Zhang J., Li C., Meng F., Guan Y., Zhang T., Yang B., Ren Z., Liu X., Li D., Zhao J., Zhao J., Wang Y, & Peng J. (2022). Functional tissue-engineered microtissue formed by self-aggregation of cells for peripheral nerve regeneration. Stem Cell Research & Therapy, 13, 3.

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