In the case of Western blot analysis, a mouse monoclonal anti-actin antibody and POD-labelled anti-mouse antibody {from Sigma (St Louis, MO)}, were used. A rabbit polyclonal anti-HDAC7 antibody was from Euromedex (Souffelweyersheim, France), POD-labelled anti-rabbit antibody was from Cell Signaling (Beverly, MA). DMEM cell culture media, penicillin, streptomycin, trypsin-EDTA, hygromycin B and neomycin were from InVitrogen(Carlsbad, NM)To conduct the immunostaining, mouse monoclonal antibody (mAb) anti-HDAC7 (20 µg/mL, Sigma-Aldrich, France), and a rabbit anti-Nur77 antibody (Ab) (5 µg/mL, Thermo Scientific, CergyPontoise, France) were used. Biotin-conjugated F(ab')2 fragment of goat antibodies to mouse IgG (Beckman Coulter, Roissy CDG, France) or biotin-conjugated goat anti-rabbit IgG (Sigma-Aldrich) for HDAC7 and Nur77 immunostaining respectively were used.
Biotin conjugated goat anti rabbit igg
Manufactured by Merck Group
Sourced in France
Biotin-conjugated goat anti-rabbit IgG is a secondary antibody used in various immunological techniques. It is produced by immunizing goats with rabbit immunoglobulin G (IgG) and then conjugating the resulting antibodies with biotin, a small molecule that can be detected using streptavidin-based detection systems.
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Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Dmem cell culture media, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Anti actin antibody, by Merck Group (1 mentions)
Neomycin, by Thermo Fisher Scientific (1 mentions)
Hygromycin b, by Thermo Fisher Scientific (1 mentions)
Psd95, by Abcam (1 mentions)
Vt1000, by Leica (1 mentions)
Alexa fluor 488 or 594 secondary antibody, by Thermo Fisher Scientific (1 mentions)
Synaptophysin, by Merck Group (1 mentions)
2 protocols using biotin conjugated goat anti rabbit igg
1
Western Blot and Immunostaining Protocol
2
Immunohistochemical Analysis of Mouse and Human Brain
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Mouse brains were fixed with 4% paraformaldehyde at 4°C for 2 days after saline perfusion. Fixed brains were cut into 30-μm-thick sections on a Vibratome (Leica VT1000S). Precut free-floating temporal cortex sections (40 µm thick) of postmortem human brains were obtained from Banner Sun Health Institute (Sun City, AZ) for immunohistochemistry. The following primary antibodies were used for immunostaining: CypD (Cat# ab110324; Abcam), MAP2 (Cat# 1281959; Boehringer Mannheim), PSD95 (Cat# 16495; Abcam), synaptophysin (Cat# MAB5258; Chemicon), synapsin I (Cat# PA1-4673; Affinity Bioreagents), anti–4-hydroxy-2-nonenal (4-HNE) IgG (1:100, Abcam). Alexa Fluor 488 or 594 secondary antibodies (Invitrogen) or biotin-conjugated goat anti-rabbit IgG (Sigma-Aldrich) were used. Sections were imaged under a Leica DMI4000B confocal microscope, and images were analyzed by the Universal MetaMorph Image Program (Molecular Devices).
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