Ecl chemiluminescence method

Manufactured by Bio-Rad

ECL (Enhanced Chemiluminescence) is a detection method used in various laboratory applications, such as Western blotting, to visualize and quantify proteins. The ECL method involves the use of chemiluminescent substrates that emit light when they react with the enzyme-labeled target proteins. This light emission is then detected and measured, providing a way to analyze the presence and relative abundance of specific proteins in a sample.

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Lab products found in correlation

Protease inhibitor cocktail, by Roche (2 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions)

Close spelling variants of this product

Enhanced chemiluminescence (ECL) detection method Chemiluminescence method ECL method ECL chemiluminescence

2 protocols using ecl chemiluminescence method

GFP Pull-Down Assay and Western Blot Analysis

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GFP pull-down assay was performed as previously described^{32 (link)}.
For WB analysis, cells were lysed in RIPA buffer (1% Triton X-100, 0.1% SDS, 150 mM NaCl, 1 mM EDTA pH 8, 10 mM Tris-HCl pH 8, and 1% protease inhibitor cocktail (Roche, Italy) centrifuged for 15 min at 4 °C and heated for 5 min at 95 °C; 25 µg of protein extract was subjected to SDS-polyacrylamide gel electrophoresis, blotted on PVC membrane (Bio-Rad, USA) and incubated overnight with appropriate antibodies. Relative protein expression was detected by ECL Chemiluminescence method (Bio-Rad). Bands intensity were quantified by Image J analysis.

Del Gaudio N., Di Costanzo A., Liu N.Q., Conte L., Migliaccio A., Vermeulen M., Martens J.H., Stunnenberg H.G., Nebbioso A, & Altucci L. (2019). BRD9 binds cell type-specific chromatin regions regulating leukemic cell survival via STAT5 inhibition. Cell Death & Disease, 10(5), 338.

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Western Blot Analysis of Protein Expression

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WB analysis was performed as previously reported [12 (link)]. Briefly, cells were lysed in RIPA buffer (1% Triton X-100, 0.1% SDS, 150 mM NaCl, 1 mM EDTA pH 8, 10 mM Tris-HCl pH 8) containing 1% protease inhibitor cocktail (Roche). Cell extract was centrifuged for 15 min at 4 °C and heated for 5 min at 95 °C; 30 μg of total protein extract was subjected to SDS-polyacrylamide gel electrophoresis, blotted on nitrocellulose membrane (Bio-Rad) and incubated ON with the specific antibodies. Protein expression was detected by ECL chemiluminescence method (Bio-Rad).

Del Gaudio N., Di Costanzo A., Liu N.Q., Conte L., Dell’Aversana C., Bove G., Benedetti R., Montella L., Ciardiello F., Carafa V., Ambrosino C., Tucci V., Conte M., Martens J.H., Stunnenberg H.G., Nebbioso A, & Altucci L. (2022). CBX2 shapes chromatin accessibility promoting AML via p38 MAPK signaling pathway. Molecular Cancer, 21, 125.

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