Protein quantitative kit

Rat immortalized myocardial H9C2 cell line was purchased from the Shanghai cell bank of Chinese Academy of Sciences; kaempferol was from Sigma-Aldrich Company (United States of America [USA]); cell counting kit 8 (CCK8) was obtained from Qihai Biology Company (Shanghai, China); Dulbecco’s Modified Eagle’s Medium (DMEM), trypsin, penicillin, and streptomycin from Hyclone Company (USA); fetal bovine serum, protein quantitative kit, and Lipofectamin 3000 transfection reagent were collected from Thermo Fisher Scientific Inc. (USA); SIRT3 short interfering ribonucleic acid (siRNA) was designed and synthesized by Gemma company (Shanghai, China); cell culture bottles, Petri dishes, and centrifuge tubes were brought from Corning company (USA); SIRT3, SOD2, B-cell lymphoma 2 (Bcl2), BCL2-associated X protein (Bax), and β-actin antibodies were from Cell Signaling Technology (USA); goat anti-mouse and goat anti-rabbit second antibodies were obtained from Zhongshan Jinqiao Biological Company (Beijing, China); reactive oxygen species (ROS), glutathione (GSH), and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase kit were purchased from Nanjing Jiancheng Institute of Bioengineering (Nanjing, China); and Western blot equipment, light-emitting photography system was purchased from Bio-Rad Company (USA).