Cellulase was supported by Dalian Meilun Biological Technology Co., Ltd (Dalian, China). DEAE Sepharose™ fast flow was purchased from GE Healthcare Life Science (Piscataway, NJ, USA). Phenol, potassium persulfate, 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Sodium hydroxide, ethanol, chloroform, N-butanol, sodium chloride and sulfuric acid were of analytical grade and were obtained from Guangzhou Reagent Co., Ltd (Guangzhou, China). 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) was purchased from Sigma Life Science (St. Louis, MO, USA). Rhamnose, l -arabinose, l -fucose, dl -xylose, d -mannose, d -glucose, and d -galactose were purchased from Shanghai Yuanye Bio-Technology Co. Ltd (Shanghai, China). All other chemicals and reagents used were of analytical grade.
1 1 diphenyl 2 picrylhydrazyl
Manufactured by Maclin Biochemical Technology
Sourced in China
1,1-diphenyl-2-picrylhydrazyl is a stable free radical compound commonly used as a reagent in various analytical and experimental applications. It serves as a tool for the measurement and evaluation of antioxidant properties in chemical systems.
Automatically generated - may contain errors
Lab products found in correlation
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (1 mentions)
L arabinose, by Yuanye Bio-Technology (1 mentions)
D mannose, by Yuanye Bio-Technology (1 mentions)
D galactose, by Yuanye Bio-Technology (1 mentions)
Phenol, by Maclin Biochemical Technology (1 mentions)
D glucose, by Yuanye Bio-Technology (1 mentions)
Potassium persulfate, by Maclin Biochemical Technology (1 mentions)
Deae sepharose fast flow, by GE Healthcare (1 mentions)
Rhamnose, by Yuanye Bio-Technology (1 mentions)
Cellulase, by Meilun (1 mentions)
Diammonium salt, by Maclin Biochemical Technology (1 mentions)
Folin phenol reagent, by Sinopharm (1 mentions)
Feso4, by Sinopharm (1 mentions)
K2s2o8, by Sinopharm (1 mentions)
1 anilino 8 naphtha lene sulfonate, by Maclin Biochemical Technology (1 mentions)
Ethylenediaminetetraacetic acid disodium salt, by Sinopharm (1 mentions)
Trichloroacetic acid, by Sinopharm (1 mentions)
Potassium ferricyanide, by Sinopharm (1 mentions)
Polycaprolactone, by Maclin Biochemical Technology (1 mentions)
Anhydrous ethanol, by Sinopharm (1 mentions)
6 protocols using 1 1 diphenyl 2 picrylhydrazyl
1
Cellulase Enzyme Characterization
2
Antioxidant and Antiglycation Assays
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BSA and gallic acid were provided by the China National Pharmaceutical Group. Glu, FeSO4, 0.01% H2O2, K2S2O8, Folin phenol reagent, potassium ferricyanide, ethylenediaminetetraacetic acid disodium salt (EDTA), and trichloroacetic acid (TCA) were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Lactic acid bacteria (LAB) preserved in a laboratory, arginine (Arg), glyoxal (GO) (40% aqueous solution), Methylglyoxal (40% aqueous solution), aminoguanidine, diammonium salt (ABTS), 1-anilino-8-naphthalene sulfonate (ANS), 1,1-diphenyl-2-picrylhy-drazyl (DPPH), and ortho-phthalaldehyde (OPA) were purchased from Macklin Biochemical Co. (Shanghai, China). In addition, ο-phenylenediamine (ο-PDA), nitroblue tetrazolium (NBT), 2,4-dinitrophenylhydrazine (2,4-DNPH), 1,10-phenanthroline, and 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) were purchased from Aladdin (Shanghai, China). All reagents were of analytical grade.
3
Synthesis and Evaluation of Tea Tree Oil-based Antimicrobial Composites
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Australian tea tree leaves were supplied by Dashan Seedling Farm (Zhangpu County, Fujian, China). Tea tree oil was obtained from Jiangxi Kangshengtang Pharmaceutical Industry Co., Ltd. (Ji’an, China). Polyvinylpyrrolidone (PVP, MW = 1,300,000), polycaprolactone (PCL, MW = 14,000), 1,1-diphenyl-2-picryl-hydrazyl (DHPP), and ferrous sulfate heptahydrate (AR) were all supplied by Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Hydrogen peroxide solution (30 wt%) was purchased from Xilong Chemical Co., Ltd. (Shantou, China). Anhydrous ethanol (AR) was supplied by Sinopharm Chemical Reagent Co., Ltd., (Shanghai, China). Potassium ferricyanide (AR), ferric chloride hexahydrate (AR), and trichloroacetic acid (AR) were provided by Guangdong Guanghua Technology Co., Ltd., (Guangzhou, China). Escherichia coli (E. coli, CMCC(B) 44103) and Staphylococcus aureus (S. aureus, ATCC 25923) were all obtained from China Center of Industrial Culture Collection (Beijing, China). Tryptic soy agar (TSA) and tryptone soya broth (TSB) were obtained from Beijing Land Bridge Technology Co., Ltd., (Beijing, China).
4
Characterization of Polysaccharide Properties
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Sephadex G-200 and DEAE cellulose-52 were purchased from Kuer Co., Ltd. (Hefei, China). Trifluoroacetic acid (TFA), hydrogen peroxide and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). The National Institute for Food and Drug Control (Beijing, China) provided dextrans of a series of molecular weights (Mw: 1, 5, 12, 50, and 150 kDa). Sigma-Aldrich provided the monosaccharide standards for rhamnose (Rha), arabinose (Ara), fructose (Fru), galactose (Gal), glucose (Glc), xylose (Xyl), mannose (Man), galacturonic acid (GalA), and glucuronic acid (GlcA). Fetal bovine serum (FBS), Phosphate Buffered Saline (PBS), Dulbecco’s modified Eagle medium (DMEM), Cell Counting Kit-8, 4% Paraformaldehyde solution, Trypsin-EDTA and Crystal Violet Staining Solution, human TNF-α, Annexin V-FITC Apoptosis Detection Kit, Cell Cycle Staining kits, Human interleukin-1β ELLSA Kit (IL-1β), Human interleukin-10 ELLSA Kit (IL-10) and Human interleukin-6 ELLSA Kit (IL-6) were purchased from different manufacturers.
5
Antioxidant Capacity Evaluation of Chitosan and Pectin
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Chitosan (deacetylation ≥ 90%) and pectin (purity ≥ 65.0%) were purchased from Yuanye (Shanghai, China). 2,2′-azinobis-3-ethylbenzthiazoline-6-sulphonate (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were obtained from Macklin (Shanghai, China). Gallic acid (purity ≥ 98%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Curcumin was obtained from Aladdin (Shanghai, China). Fresh pork was purchased from a local supermarket in Guangzhou, China. All other reagents used were analytic grade.
6
Grape Antioxidant Extraction Protocol
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GS was obtained from fresh grapes (Vitisvinifera cv. Kyoho). PVA (AR > 97%) was supplied by the Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). 1,1-diphenyl-2-picrylhydrazyl (DPPH) and sodium hydroxide (NaOH) were purchased from Shanghai Macklin Biochemical Technology Co., Ltd.
(Shanghai, China). Ethanol (C 2 H 5 OH) and methanol (CH 3 OH) were provided by Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Hydrochloric acid (HCl), magnesium nitrate (Mg(NO 3 ) 2 ), and potassium nitrate (KNO 3 ) were purchased from Damao Chemical Reagent Factory (Tianjin, China).
(Shanghai, China). Ethanol (C 2 H 5 OH) and methanol (CH 3 OH) were provided by Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Hydrochloric acid (HCl), magnesium nitrate (Mg(NO 3 ) 2 ), and potassium nitrate (KNO 3 ) were purchased from Damao Chemical Reagent Factory (Tianjin, China).
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