Iba1 antibody

Brain tissues were sectioned on a standard rotary microtome (Leica, Berlin, Germany). Tissue sections were collected and counterstained with hematoxylin-eosin (HE). Then, the sections were subjected to staining with the primary antibodies. Primary antibodies were tyrosine hydroxylase (TH, 1:1000) (Santa Cruz Biotech., Dallas, TX, USA), P-CREB (1:500, Upstate Comp., Lake Placid, NY, USA), caspase 3 (1:500), or MDA (1:100). After raining with PBS, sections were stained with 3,3′-diaminobenzidine (DAB) as described [24 (link)]. Subsequently, the selected sections were further performed by using Tuj1 (1:500), GFAP (1:500), and IBA1 antibody (1:200, Santa Cruz Biotech., Dallas, TX, USA). The stained sections were counterstained with DAPI, mounted and examined microscopically.

MKP-1 (#sc-373841), p-p38 (#sc-166182), p38 (#sc-81621), p-JNK (#sc-6254), JNK (#sc-7345), p-ERK (#sc-7383), ERK (#sc-135900), BDNF (#sc-65514), Synapsin Ia (#sc-376623), MBP (#sc-376995), Bax (#sc-20067), Bcl-2 (#sc-7382) antibodies were purchased from Santa cruz biotechnology (United States). Goat anti-rabbit IgG/horseradish enzyme labeling (#16K22C) was purchased from Boster Biological Technology (Wuhan, China), and goat anti-mouse IgG/horseradish enzyme labeling (#ZB-2305) was purchased from Zhongshanjinqiao biotechnology (Beijing, China). β-actin antibody (bsm-33036m) (1:1000) was purchased from Beijing Boaosen Biotechnology Co., Ltd. (Beijing, China). L-glutamine (G8540) was purchased from Sigma-Aldrich (United States). Rat IL-1β (SBJ-H0417), IL-6 (SBJ-H0465), TNF-α (SBJ-H0038) ELISA kit reagent kits were purchased from Nanjing Sempega Biotechnology Co. (Nanjing, China). ROS (MM-43700M2), GSH-PX (MM-20251R2), SOD (MM-20387R2), MDA (MM-0385R1) ELISA kits were purchased from Jiangsu Meimian Industrial Co., Ltd. (Yancheng, China). TUNEL kit was purchased from Promega (United States). H-E staining kit was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). Iba-1 antibody was purchased from Santa cruz biotechnology (United States). DAB kit was purchased from Beijing Boaosen Biotechnology Co., Ltd. (Beijing, China).