Se methyl selenocysteine hydrochloride

Myc-CaP, an androgen sensitive mouse prostate cancer cell line, is derived from c-Myc transgenic mouse. LNCaP (a human androgen sensitive prostate carcinoma cell line), DU145 and PC3 cell lines are purchased from American Type Culture Collection (ATCC). These cell lines were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, and incubated in a humidified incubator with 5% CO₂ at 37 °C. Myc-CaP cells and DU145 cells stably expressing control shRNA (Sigma), INMT shRNA (Sigma) or SMYD3 shRNA (Sigma) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and puromycin (10 μg/mL). Myc-CaP cells engineered to stably express control vector and pCMV-INMT were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and hygromycin (100 μg/mL). DMT was purchased from Cayman Chemical company, bis(7)-Tacrine, BCl-121, Methylseleninic acid (MSA), and Se-(Methyl)selenocysteine hydrochloride (MSC) were purchased from Sigma.

Folin–Ciocalteu reagent
and nitric acid were purchased from VWR International (Barcelona,
Spain); methanol, acetone, sodium carbonate, and Trolox standard were
purchased from Fisher Scientific (Madrid, Spain); hydrogen peroxide
was purchased from Panreac Applichem (Barcelona, Spain); gallic acid,
diphenyl-2,4,6-trinitrophenyl iminoazanium (DPPH), sodium selenite,
sodium selenate, seleno-l-methionine (≥98%), seleno-l-cystine (95%), and Se-(Methyl) selenocysteine hydrochloride
(≥95%) were purchased from Sigma-Aldrich (St. Louis); and Milli-Q
water was purified through a purification system from Millipore (Billerica,
MA).

Potassium dihydrogen phosphate, di-potassium hydrogen phosphate, EDTA, sodium hydroxide, aminooxyacetic acid (AOAA), l-tryptophan (l-Try), indole-3-pyruvic acid (IPA), phenylpyruvic acid (PPA), α-Keto-γ-methylthiobutyric acid sodium salt (KMB), se-methylselenocysteine hydrochloride (MSC), dimethyl-2-oxoglutarate (α-KG), l-phenyl alanine (l-Phe), 2-amino-2-methyl-1,3-propanediol, pyridoxal 5′-phosphate hydrate (PLP), 2,4-dinitrophenylhydrazine (DNPH), Phenylmethanesulfonyl fluoride (PMSF), RIPA buffer, protease inhibitor cocktail mix, and N-N-dimethyl formamide, pLKO.1 vector were purchased from Sigma-Aldrich (Darmstadt, Germany). BFF-122 was purchased from Axon Medchem (Groningen, Netherlands), NADPH was purchased from Acros Organics (New Jersey, NJ, USA). Plasmid pEGFP-N1 (Clontech, Takara Bio USA, Inc, Mountain View, CA, USA) was a generous gift from Dr. Gildert Lauter from the Department for Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden. HEPG2 cells and EMEM media were purchased from ATCC (Wesel, Germany).