Deionized water was purified with a Millipore Milli-Q RG water purification system (Darmstadt, Germany). Dithiothreitol and iodoacetamide were purchased from Thermo Fisher Scientific (Waltham, MA) and freshly prepared in 50 mM ammonium bicarbonate buffer each day before use. MS-grade trypsin and Lys-C were products of Promega (Madison, WI). Unless otherwise mentioned, all other reagents were obtained from Sigma-Aldrich (St. Louis, MO).
Milli q rg water purification system
Manufactured by Merck Group
Sourced in United States
The Milli-Q RG water purification system is a laboratory equipment designed to produce ultrapure water. It utilizes a multi-stage purification process to remove impurities, resulting in high-quality water suitable for various research and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ms grade trypsin, by Promega (1 mentions)
Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions)
Iodoacetamide, by Thermo Fisher Scientific (1 mentions)
Lys c, by Promega (1 mentions)
Solvents hplc grade, by Merck Group (1 mentions)
Dmso d6, by Cambridge Isotopes (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Oleic acid, by Tokyo Chemical Industry (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Thiourea, by Merck Group (1 mentions)
Na2b4o7 10h2o, by Avantor (1 mentions)
Nah2po4, by Carlo Erba (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Thiourea, by Avantor (1 mentions)
4 protocols using milli q rg water purification system
1
Protein Purification and Digestion
2
Antioxidant Activity Evaluation Protocol
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DMSO was purchased from Fisher (# D128‐1). D‐α‐Tocopherol (# T2309), DL‐α‐Tocopherol (# T0251), and DL‐α‐Tocopherol Acetate (# T0252) were purchased from TCI. CTX was purchased from Carbosynth (# FC19621). Phosphate buffer saline (PBS) at pH 7.4 and solvents (HPLC grade) were purchased from Merck (Darmstadt, Germany). The aqueous solutions were prepared using ultrapure water (Milli‐Q RG water purification system, Millipore, Burlington, MA, USA). DMSO‐d6 was purchased from Cambridge Isotope Laboratories, Inc.
3
Preparation and Characterization of IMQ-Loaded Lipid Formulations
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Imiquimod (IMQ, purity > 98%, molar mass 240.31 g/mol) and oleic acid (OA, >99%) were purchased from TCI chemicals (Tokyo, Japan). Phospholipids (PL) from egg yolk (60% phosphatidylcholine, mostly 16:0/18:2, 15% phosphatidylethanolamine, mostly 18:0/18:2 and 18:0/20:4, minor fractions phosphatidylinositol, lysophosphatidylcholine, sphingomyelin, lysophosphatidylethanolamine), acetic acid (>99%), ethanol (>99%), acetonitrile (HPLC grade), and methanol (HPLC grade) were purchased from Sigma Aldrich (Schnelldorf, Germany). Aldara topical cream (5% IMQ) MEDA company, Sweden was obtained from Hospital Pharmacy, Hradec Králové, Czech Republic. Water was deionized, distilled, and filtered through a Milli-Q RG water purification system (Millipore, Burlington, MA, USA).
4
Cyclodextrin Capillary Electrophoresis Analysis
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All chemical and solvents used were of analytical grade or of HPLC quality.
β-CD was supplied by Sigma (Saint-Louis, MO, USA). α-CD, γ-CD, and dimethyl-β-CD (Me-β-CD) were purchased from Acros Organics (Fisher Scientific, IIIkirch, France) and 2-hydroxypropyl-β-CD (HP-β-CD) was from Cyclo Lab LTD (Budapest, Hungary). The tetraborate sodium buffer (10 mM, pH = 9.5) was prepared from Na2B4O7.10H2O (VWR, Fontenay-sous-Bois, France). Different concentrations of CDs were prepared with this tetraborate buffer for ACE experiments. The phosphate buffer (40 mM, pH = 7.0) was prepared by mixing appropriate amounts of NaH2PO4 (Carlo Erba, Val-de-Reuil, France) and Na2HPO4
(Sigma, Saint-Louis, MO, USA).
A thiourea solution at 0.01% (w/w) in water was used as an electroosmotic flow (EOF) marker. thiourea was from VWR (Fontenay-sous-Bois, France).
A MilliQ RG water purification system (Millipore, Bedford, MA, USA) was used to provide ultrapure water (18.2 MΩ.cm).
All buffers were filtered through a 0.20 µm nylon membrane from VWR supplier.
β-CD was supplied by Sigma (Saint-Louis, MO, USA). α-CD, γ-CD, and dimethyl-β-CD (Me-β-CD) were purchased from Acros Organics (Fisher Scientific, IIIkirch, France) and 2-hydroxypropyl-β-CD (HP-β-CD) was from Cyclo Lab LTD (Budapest, Hungary). The tetraborate sodium buffer (10 mM, pH = 9.5) was prepared from Na2B4O7.10H2O (VWR, Fontenay-sous-Bois, France). Different concentrations of CDs were prepared with this tetraborate buffer for ACE experiments. The phosphate buffer (40 mM, pH = 7.0) was prepared by mixing appropriate amounts of NaH2PO4 (Carlo Erba, Val-de-Reuil, France) and Na2HPO4
(Sigma, Saint-Louis, MO, USA).
A thiourea solution at 0.01% (w/w) in water was used as an electroosmotic flow (EOF) marker. thiourea was from VWR (Fontenay-sous-Bois, France).
A MilliQ RG water purification system (Millipore, Bedford, MA, USA) was used to provide ultrapure water (18.2 MΩ.cm).
All buffers were filtered through a 0.20 µm nylon membrane from VWR supplier.
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