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Optometry

Manufactured by Cerebral Mechanics
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The OptoMetry is a laboratory equipment designed for optical measurement and analysis. It features advanced optics and sensors to gather precise data on various optical properties. The core function of the OptoMetry is to provide accurate and reliable optical measurements for research and development applications.

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OptoMetry system Optometry software and apparatus Virtual Optometry System

3 protocols using optometry

1

Automated Visual Function Assessment in Diabetic Mice

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The virtual optokinetic system (OptoMetry; CerebralMechanics, Inc,) was used as described previously [19 ,27 (link)]. At 14 weeks of diabetes, mice were allowed to move freely on the platform of the visual OptoMotor system while the visual stimulus was projected around the platform. Spatial frequency threshold was measured at maximum contrast. For contrast sensitivity, only a single value (0.064 c/d; the maximal point in a full contrast sensitivity curve) was measured. These tests are psychophysical measures that assess function of both retinal and central visual pathways. Each measurement was repeated several times to evaluate the reproducibility of responses. The grader was masked with respect to the animals’ experimental group.
Saliba A., Du Y., Liu H., Patel S., Roberts R., Berkowitz B.A, & Kern T.S. (2015). Photobiomodulation Mitigates Diabetes-Induced Retinopathy by Direct and Indirect Mechanisms: Evidence from Intervention Studies in Pigmented Mice. PLoS ONE, 10(10), e0139003.
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2

Spatial Frequency OKR Assessment

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Visual function was assessed by measuring the OKR using commercial software and apparatus (OptoMetry; CerebralMechanics, Inc., Medicine Hat, AB, Canada) as previously described(15 (link)). OKR was determined as the highest spatial frequency where mice track a 100% contrast grating that is projected at different spatial frequencies. Measurements were performed by an investigator masked to the experimental treatments. Each data point represents 10 animals in experiments performed in triplicate.
Ross A.G., McDougald D.S., Khan R.S., Duong T.T., Dine K.E., Aravand P., Bennett J., Chavali V.R, & Shindler K.S. (2021). Rescue of retinal ganglion cells in optic nerve injury using cell-selective AAV mediated delivery of SIRT1. Gene Therapy, 28(5), 256-264.
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3

Measuring Visual Function via OKR

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Visual function was assessed by measuring the OKR using commercial software and apparatus (OptoMetry; CerebralMechanics, Inc., Medicine Hat, AB, Canada) as previously described [15 (link)]. OKR was determined as the highest spatial frequency where mice track a 100% contrast grating that is projected at different spatial frequencies. Measurements were performed by an investigator masked to the experimental treatments. Each datapoint represents ten animals in experiments performed in triplicate.
Ross A.G., McDougald D.S., Khan R.S., Duong T.T., Dine K.E., Aravand P., Bennett J., Chavali V.R, & Shindler K.S. (2021). Rescue of retinal ganglion cells in optic nerve injury using cell-selective AAV mediated delivery of SIRT1. Gene Therapy, 28(5), 256-264.
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