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P 4e bp

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P-4E-BP is a protein that plays a role in the regulation of protein synthesis. It functions as a repressor of the translation initiation factor eIF4E, which is involved in the recruitment of ribosomes to mRNA.

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Lab products found in correlation

Ab2606, by Abcam (2 mentions) Fluorescent secondary antibody, by LI COR (2 mentions) Fluorescent total protein stain, by LI COR (2 mentions) 4e bp, by Abcam (2 mentions) Ab 27792, by Santa Cruz Biotechnology (2 mentions) Sc 11386, by Abcam (2 mentions) Ab32157, by Proteintech (2 mentions) P eif2α, by Abcam (2 mentions) Eif2α, by Santa Cruz Biotechnology (2 mentions) P akt2, by Abcam (1 mentions) T mtor, by Santa Cruz Biotechnology (1 mentions) P akt1, by Abcam (1 mentions) P s6k, by Abcam (1 mentions) P mtor, by Abcam (1 mentions)

3 protocols using p 4e bp

1

Western Blot Analysis of SMA Mice

Cited 1 time
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Western blot on tissues from SMA and control mice was performed exactly as described before (64 (link)). The following primary antibodies were used 4E-BP (Abcam; Ab-2606 (1:1000)); P-4E-BP (Abcam; Ab-27792 (1:1000)); eIF2α (Santa Cruz Biotechnology; Sc-11386 (1:1000)); p-eIF2α (Abcam; Ab32157 (1:1000)); Get4 (Proteintech 27768-1-AP (1:1000)); AE2 (acetylcholinesterase) (DSHB AE2DSHB (1:1000)). Antibody detection was performed using fluorescent secondary antibodies (LI-COR) and protein loading was normalized to a fluorescent total protein stain (LI-COR). Quantification was performed as described previously (34 (link)).
Lauria F., Bernabò P., Tebaldi T., Groen E.J., Perenthaler E., Maniscalco F., Rossi A., Donzel D., Clamer M., Marchioretto M., Omersa N., Orri J., Serra M.D., Anderluh G., Quattrone A., Inga A., Gillingwater T.H, & Viero G. (2020). SMN-primed ribosomes modulate the translation of transcripts related to Spinal Muscular Atrophy. Nature cell biology, 22(10), 1239-1251.
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2

Western Blot Analysis of SMA Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot on tissues from SMA and control mice was performed exactly as described before (64 (link)). The following primary antibodies were used 4E-BP (Abcam; Ab-2606 (1:1000)); P-4E-BP (Abcam; Ab-27792 (1:1000)); eIF2α (Santa Cruz Biotechnology; Sc-11386 (1:1000)); p-eIF2α (Abcam; Ab32157 (1:1000)); Get4 (Proteintech 27768-1-AP (1:1000)); AE2 (acetylcholinesterase) (DSHB AE2DSHB (1:1000)). Antibody detection was performed using fluorescent secondary antibodies (LI-COR) and protein loading was normalized to a fluorescent total protein stain (LI-COR). Quantification was performed as described previously (34 (link)).
Lauria F., Bernabò P., Tebaldi T., Groen E.J., Perenthaler E., Maniscalco F., Rossi A., Donzel D., Clamer M., Marchioretto M., Omersa N., Orri J., Serra M.D., Anderluh G., Quattrone A., Inga A., Gillingwater T.H, & Viero G. (2020). SMN-primed ribosomes modulate the translation of transcripts related to Spinal Muscular Atrophy. Nature cell biology, 22(10), 1239-1251.
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3

Western Blot Analysis of Signaling Proteins

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The expression levels of T-Akt1/2, T-Akt1, T-Akt2, T-mTOR, T-eukaryotic translation initiation factor 4E binding protein (4eBP), T-ribosomal protein s6 kinase (S6K) T-p65, T-p38, IκBα, arginine enzyme 1 (Arg-1) (Santa Cruz Biotechnology, USA), P-Akt1/2, P-Akt1, P-Akt2, P-mTOR, P-4E-BP, P-p38, P-p65, and P-S6K (Abcam, U.K.) were detected by western blot following the manufacturer's instructions.
Zhao Z., Pan G., Tang C., Li Z., Zheng D., Wei X, & Wu Z. (2018). IL-34 Inhibits Acute Rejection of Rat Liver Transplantation by Inducing Kupffer Cell M2 Polarization. Transplantation, 102(6).
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