Cytokine and chemokine levels were determined in undiluted BAL fluid supernatant using the V-PLEX® Proinflammatory and Cytokine Panel 1 (mouse) Kit MSD® Multi-Spot Assay System (Meso Scale Diagnostics, LLC), according to protocol. Absorbance was measured on the Meso Scale Discovery (MSD) plate reader (Meso Scale Diagnostics, Maryland, USA). The following cytokines and chemokines were included in the panel: IL-4, IL-10, IFN-γ, IL-2, IL-5, KC/GRO, IL-1β, IL-12p70, TNF-α, IL-6, IL-15, IP-10, MCP-1, MIP-1α, IL-9, IL-17 A/F, IL-33, IL-27p28/IL-30 and MIP-2. Detection limits can be found in Additional File 4.
V plex proinflammatory and cytokine panel 1 mouse kit msd multi spot assay system
Manufactured by Mesoscale
The V-PLEX® Proinflammatory and Cytokine Panel 1 (mouse) Kit is a multiplex immunoassay designed for the simultaneous quantitative measurement of multiple mouse protein biomarkers in a single small-volume sample using the MSD® Multi-Spot Assay System. The kit provides a convenient and sensitive way to analyze a panel of mouse inflammatory and cytokine proteins.
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Lab products found in correlation
2 protocols using v plex proinflammatory and cytokine panel 1 mouse kit msd multi spot assay system
1
Comprehensive Cytokine and Chemokine Profiling
2
Multiplex Cytokine Profiling in BAL
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Cytokine and chemokine levels were determined in undiluted BAL fluid supernatant using the V-PLEX® Proinflammatory and Cytokine Panel 1 (mouse) Kit MSD® Multi-Spot Assay System (Meso Scale Diagnostics, LLC), according to protocol. Absorbance was measured on the Meso Scale Discovery (MSD) plate reader (Meso Scale Diagnostics, Maryland, USA). The following cytokines and chemokines were included in the panel: IL-4, IL-10, IFN-γ, IL-2, IL-5, KC/GRO, IL-1β, IL-12p70, TNF-α, IL-6, IL-15, IP-10, MCP-1, MIP-1α, IL-9, IL-17A/F, IL-33, IL-27p28/IL-30 and MIP-2. Detection limits can be found in Additional File 4.
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